Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22869 | 5' | -53.7 | NC_005137.2 | + | 108393 | 0.66 | 0.934976 |
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Target: 5'- aGCCcaaccGGCCGcccgaguugccUGUCGUUuUGCUAAGCg -3' miRNA: 3'- aCGGuu---CCGGU-----------ACAGCAG-ACGGUUUG- -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 63972 | 0.66 | 0.918356 |
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Target: 5'- cGCCGcgauGGGCCaAUGUCGgguuaaugagcUgUGCCAAGg -3' miRNA: 3'- aCGGU----UCCGG-UACAGC-----------AgACGGUUUg -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 62001 | 0.67 | 0.87821 |
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Target: 5'- cGCCAGaauGGC---GUCG-CUGCCAAGCu -3' miRNA: 3'- aCGGUU---CCGguaCAGCaGACGGUUUG- -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 26565 | 0.68 | 0.838201 |
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Target: 5'- cGCUgcGG-CGUGUCGaCUGCCAAGa -3' miRNA: 3'- aCGGuuCCgGUACAGCaGACGGUUUg -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 25737 | 0.69 | 0.829559 |
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Target: 5'- uUGCUAcGGCCAgGaCGUUUGCCGAu- -3' miRNA: 3'- -ACGGUuCCGGUaCaGCAGACGGUUug -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 114102 | 0.69 | 0.793131 |
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Target: 5'- cGCCGAGcGCCGcGUgCGUCUGCaccGCu -3' miRNA: 3'- aCGGUUC-CGGUaCA-GCAGACGguuUG- -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 82687 | 0.73 | 0.607472 |
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Target: 5'- cGCCAAGGCCGUGcgCGaccagCUGCgCAAu- -3' miRNA: 3'- aCGGUUCCGGUACa-GCa----GACG-GUUug -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 103555 | 0.74 | 0.554689 |
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Target: 5'- cGCUGAGGCUGgugGUCuGUCcGCCAAACu -3' miRNA: 3'- aCGGUUCCGGUa--CAG-CAGaCGGUUUG- -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 34056 | 0.75 | 0.483367 |
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Target: 5'- cGCUGuuuccGGCCGUGUCGuaUCUGCCuuGCg -3' miRNA: 3'- aCGGUu----CCGGUACAGC--AGACGGuuUG- -5' |
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| 22869 | 5' | -53.7 | NC_005137.2 | + | 98790 | 0.97 | 0.020176 |
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Target: 5'- uUGCCAAGGCCAU-UCGUCUGCCAAACg -3' miRNA: 3'- -ACGGUUCCGGUAcAGCAGACGGUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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