Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22874 | 5' | -52.6 | NC_005137.2 | + | 23705 | 0.73 | 0.614164 |
Target: 5'- --aACUAA-CCGGCCAAUCCACAAa- -3' miRNA: 3'- caaUGGUUcGGCCGGUUGGGUGUUaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 26935 | 0.71 | 0.772264 |
Target: 5'- ----aCGAGCCGGCCAGCaCgACGAa- -3' miRNA: 3'- caaugGUUCGGCCGGUUG-GgUGUUaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 29449 | 0.69 | 0.862579 |
Target: 5'- --gGCCAAaUUGGCCAAUCCACAc-- -3' miRNA: 3'- caaUGGUUcGGCCGGUUGGGUGUuaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 37739 | 0.7 | 0.791765 |
Target: 5'- --aACCGgcGGCUGGCCGcagacCCCGCAGUg -3' miRNA: 3'- caaUGGU--UCGGCCGGUu----GGGUGUUAa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 53703 | 0.68 | 0.899607 |
Target: 5'- ----aCGAGCCGGCCGuuuuGgCCACAAc- -3' miRNA: 3'- caaugGUUCGGCCGGU----UgGGUGUUaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 59504 | 0.67 | 0.918765 |
Target: 5'- --cGCCAgacgAGCCGGCUGACgagUCGCGGUUg -3' miRNA: 3'- caaUGGU----UCGGCCGGUUG---GGUGUUAA- -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 63057 | 0.66 | 0.954199 |
Target: 5'- uGUUACCAAuGCCaaucaaGGCgGACCCgGCGAc- -3' miRNA: 3'- -CAAUGGUU-CGG------CCGgUUGGG-UGUUaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 65778 | 0.67 | 0.932374 |
Target: 5'- cGUUugCAaacgggucaaacaagGGuUCGGCCAGCCCGCuuUUa -3' miRNA: 3'- -CAAugGU---------------UC-GGCCGGUUGGGUGuuAA- -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 68440 | 0.69 | 0.846103 |
Target: 5'- ----aCGAGUCGGCgCAGCCCAUGAg- -3' miRNA: 3'- caaugGUUCGGCCG-GUUGGGUGUUaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 71709 | 0.71 | 0.731676 |
Target: 5'- -aUACCGAGUCGGCCGuggacgaccuGCCCAg---- -3' miRNA: 3'- caAUGGUUCGGCCGGU----------UGGGUguuaa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 83697 | 1.06 | 0.00644 |
Target: 5'- uGUUACCAAGCCGGCCAACCCACAAUUa -3' miRNA: 3'- -CAAUGGUUCGGCCGGUUGGGUGUUAA- -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 88837 | 0.7 | 0.791765 |
Target: 5'- --gACgCGAcGCUGGCCAACuCCACGAUc -3' miRNA: 3'- caaUG-GUU-CGGCCGGUUG-GGUGUUAa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 95479 | 0.68 | 0.885539 |
Target: 5'- uGUUGCCcacGGCCGGCCGGa--ACGAUUg -3' miRNA: 3'- -CAAUGGu--UCGGCCGGUUgggUGUUAA- -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 100996 | 0.67 | 0.935536 |
Target: 5'- -cUGCCAcGCCuguccaaccgcGGCCGuacauggcaacGCCCACAAUa -3' miRNA: 3'- caAUGGUuCGG-----------CCGGU-----------UGGGUGUUAa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 103107 | 0.72 | 0.693812 |
Target: 5'- gGUUGCCGGgcgaugacccguggcGCCGccacgcgaucGCCAACCCGCAAUg -3' miRNA: 3'- -CAAUGGUU---------------CGGC----------CGGUUGGGUGUUAa -5' |
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22874 | 5' | -52.6 | NC_005137.2 | + | 129131 | 0.67 | 0.918164 |
Target: 5'- uUUACCAAaCCGGCCAuugaucacuugagGCgCCGCGAc- -3' miRNA: 3'- cAAUGGUUcGGCCGGU-------------UG-GGUGUUaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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