Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22882 | 5' | -59.1 | NC_005137.2 | + | 17474 | 0.66 | 0.789894 |
Target: 5'- gGGUGUGGgGCuGGUggugGUGCaGCCGUUGCa -3' miRNA: 3'- -CCGCGCUgCG-CUA----CGCGcUGGCAGCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 78721 | 0.66 | 0.771942 |
Target: 5'- cGGCuGCGGCuucgGCGAuaagUGCGCGGucugcCCGggCGCc -3' miRNA: 3'- -CCG-CGCUG----CGCU----ACGCGCU-----GGCa-GCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 75966 | 0.66 | 0.771942 |
Target: 5'- -uCGUGACGCGgcGCGCGuugguGCaCGgCGCa -3' miRNA: 3'- ccGCGCUGCGCuaCGCGC-----UG-GCaGCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 99937 | 0.66 | 0.771942 |
Target: 5'- --gGCGACGCGcacgcAUGUuggacaacggccGCGGCCG-CGCg -3' miRNA: 3'- ccgCGCUGCGC-----UACG------------CGCUGGCaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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