Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22882 | 5' | -59.1 | NC_005137.2 | + | 28050 | 0.76 | 0.253154 |
Target: 5'- uGGCGCGACGCgcgcuggGAUGCGCcagacGGCUGUacaCGCa -3' miRNA: 3'- -CCGCGCUGCG-------CUACGCG-----CUGGCA---GCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 31038 | 0.79 | 0.17693 |
Target: 5'- uGGCGCacgaagaGGCGCGcgGcCGCGGCCGUUGUc -3' miRNA: 3'- -CCGCG-------CUGCGCuaC-GCGCUGGCAGCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 45091 | 0.8 | 0.163562 |
Target: 5'- cGCGCGAcuugcucugccacuCGCGGgcGCGCGACgCGUCGCa -3' miRNA: 3'- cCGCGCU--------------GCGCUa-CGCGCUG-GCAGCG- -5' |
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22882 | 5' | -59.1 | NC_005137.2 | + | 67361 | 1.12 | 0.001038 |
Target: 5'- gGGCGCGACGCGAUGCGCGACCGUCGCc -3' miRNA: 3'- -CCGCGCUGCGCUACGCGCUGGCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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