miRNA: 3'- cCGCGCUGcGCuACG---CGCugGCAGCG- -5'
miRNA: 3'- cCGCGCUGCgCUACGCG--------------CugGCaGCG- -5'
miRNA: 3'- -CCGCG--------CUGCG--CUa--CGCGCU-GGCaGCG- -5'
miRNA: 3'- -CCGCG--------CUGCGCU--ACGCGCuGGcaGCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22882 | 5' | -59.1 | NC_005137.2 | + | 11428 | 0.67 | 0.75352 |
|
Target: 5'- aGUGUGGCaCG-UGCucaGCGugCGUCGCu -3' miRNA: 3'- cCGCGCUGcGCuACG---CGCugGCAGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 11299 | 0.73 | 0.400545 |
|
Target: 5'- uGCGCGGCGgGAUGCGUacgugcuaagcuguGugCG-CGCg -3' miRNA: 3'- cCGCGCUGCgCUACGCG--------------CugGCaGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 5871 | 0.66 | 0.78098 |
|
Target: 5'- cGGUGCauugucaaGACGUucGAgcgGCGCGGcCCGcCGCu -3' miRNA: 3'- -CCGCG--------CUGCG--CUa--CGCGCU-GGCaGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 139 | 0.7 | 0.557924 |
|
Target: 5'- cGGCGUacaauucaGGCGCGGgcUGCGCGuCCaaCGCg -3' miRNA: 3'- -CCGCG--------CUGCGCU--ACGCGCuGGcaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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