miRNA: 3'- -CCGCGCuGCGCUa----CGC-GCU---GGCAGCG- -5'
miRNA: 3'- cCGCGC-------UGCGCUa-----CGCGC-----------UGGCaGCG- -5'
miRNA: 3'- cCGCGCUG-------------CGCUaCGCgCUGG-CAGCG- -5'
miRNA: 3'- -CCGCGcugCGCUACGCGC-----------UGGCA--GCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22882 | 5' | -59.1 | NC_005137.2 | + | 84289 | 0.68 | 0.696109 |
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Target: 5'- uGGCGCG-CGUGGaaacgGUGuCGAuguCCGUUGCg -3' miRNA: 3'- -CCGCGCuGCGCUa----CGC-GCU---GGCAGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 22827 | 0.68 | 0.704881 |
|
Target: 5'- uGCGCGuugccaaACGCGAgggcggGCGCGcgcauaccgacGCCGcCGCc -3' miRNA: 3'- cCGCGC-------UGCGCUa-----CGCGC-----------UGGCaGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 99798 | 0.67 | 0.714571 |
|
Target: 5'- uGCGCGACcgcugguacaaggGCGAcGCG-GGCCgGUUGCg -3' miRNA: 3'- cCGCGCUG-------------CGCUaCGCgCUGG-CAGCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 95446 | 0.66 | 0.80731 |
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Target: 5'- uGGCGCcg-GCGGUGCGUGucuugacaagcGCCGUguUGCc -3' miRNA: 3'- -CCGCGcugCGCUACGCGC-----------UGGCA--GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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