miRNA: 3'- -CCG-CGCUGCgC----UAC-GC-GCUGGCAGcg -5'
miRNA: 3'- cCGCGCUGCGCUaCGCGCuGGCaGcg -5'
miRNA: 3'- -CCGCGCUGCG---------------CuaCGCGC--UGGCa----------GCG- -5'
miRNA: 3'- cCGCGCU-GCGCua--CGCGCuGGcAGCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22882 | 5' | -59.1 | NC_005137.2 | + | 107140 | 0.68 | 0.666611 |
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Target: 5'- aGGCgGCGGCGgGuuggGUGaCGcCGACCGUCa- -3' miRNA: 3'- -CCG-CGCUGCgC----UAC-GC-GCUGGCAGcg -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 97992 | 0.68 | 0.666611 |
|
Target: 5'- cGCGCGGCGUGG-GUGCGcCCGcCa- -3' miRNA: 3'- cCGCGCUGCGCUaCGCGCuGGCaGcg -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 125032 | 0.68 | 0.660675 |
|
Target: 5'- cGGCGUGGCGCaggcaacggagccggGcgGCGCGgcGCCGgaucugaacggCGCa -3' miRNA: 3'- -CCGCGCUGCG---------------CuaCGCGC--UGGCa----------GCG- -5' |
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| 22882 | 5' | -59.1 | NC_005137.2 | + | 112399 | 0.66 | 0.798673 |
|
Target: 5'- uGCGCGAuCGCGcacgGCGCG-CCaUUGUg -3' miRNA: 3'- cCGCGCU-GCGCua--CGCGCuGGcAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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