Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22883 | 3' | -56.4 | NC_005137.2 | + | 67042 | 1.06 | 0.0033 |
Target: 5'- aUUGGCCCAUCGCGGCGUCAAACGUGUa -3' miRNA: 3'- -AACCGGGUAGCGCCGCAGUUUGCACA- -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 34642 | 0.72 | 0.503799 |
Target: 5'- gUUGGcCCCGUCGCGGCG-UAAGCa--- -3' miRNA: 3'- -AACC-GGGUAGCGCCGCaGUUUGcaca -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 40257 | 0.71 | 0.564245 |
Target: 5'- aUUGGCCg--UGUGGCGcgCAAACGUGUu -3' miRNA: 3'- -AACCGGguaGCGCCGCa-GUUUGCACA- -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 51701 | 0.7 | 0.647282 |
Target: 5'- -cGGCaCCGUUGCGcGCGUCGGaagcgcauguACGUGc -3' miRNA: 3'- aaCCG-GGUAGCGC-CGCAGUU----------UGCACa -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 10690 | 0.69 | 0.657691 |
Target: 5'- uUUGGCCaccguuUCGUGGCGuUCGAugGCGUGc -3' miRNA: 3'- -AACCGGgu----AGCGCCGC-AGUU--UGCACa -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 44828 | 0.68 | 0.70924 |
Target: 5'- -cGGCCgCcUUGuCGGCGUCAAACGa-- -3' miRNA: 3'- aaCCGG-GuAGC-GCCGCAGUUUGCaca -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 63003 | 0.68 | 0.72945 |
Target: 5'- -cGGaCCCAUggUGCGGCG-CcGGCGUGUc -3' miRNA: 3'- aaCC-GGGUA--GCGCCGCaGuUUGCACA- -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 81675 | 0.67 | 0.778278 |
Target: 5'- aUGGCCgaAUCGCugccGGUGUCGGGCGg-- -3' miRNA: 3'- aACCGGg-UAGCG----CCGCAGUUUGCaca -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 13995 | 0.67 | 0.787674 |
Target: 5'- aUUGGCCCGaCGUGGa-UCgGGACGUGUu -3' miRNA: 3'- -AACCGGGUaGCGCCgcAG-UUUGCACA- -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 32634 | 0.67 | 0.794166 |
Target: 5'- --aGCCCGUCGCgacggcgccaaaccGGCGcaCAAGCGUGa -3' miRNA: 3'- aacCGGGUAGCG--------------CCGCa-GUUUGCACa -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 109441 | 0.66 | 0.823716 |
Target: 5'- -cGGCCCAUcggucacccaaCGCGGCGUUu--CGUc- -3' miRNA: 3'- aaCCGGGUA-----------GCGCCGCAGuuuGCAca -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 28509 | 0.66 | 0.832296 |
Target: 5'- gUGGCCCAacuugacgCGCguuucGGCGUUgauGACGUGa -3' miRNA: 3'- aACCGGGUa-------GCG-----CCGCAGu--UUGCACa -5' |
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22883 | 3' | -56.4 | NC_005137.2 | + | 73814 | 0.66 | 0.855291 |
Target: 5'- gUUGGCCaaaaacgCGUCGCGGUcacaaagGUCGgccauAACGUGUc -3' miRNA: 3'- -AACCGG-------GUAGCGCCG-------CAGU-----UUGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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