Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22885 | 3' | -53.3 | NC_005137.2 | + | 115290 | 0.66 | 0.955455 |
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Target: 5'- gAGCAGCGGAcgagCUgCGGgcgauuGCGCCGc -3' miRNA: 3'- aUCGUCGUCUaaaaGGgGCU------UGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 126351 | 0.66 | 0.937424 |
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Target: 5'- -cGCGuGCAaug-UUUCCCGGACGCUGg -3' miRNA: 3'- auCGU-CGUcuaaAAGGGGCUUGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 80371 | 0.67 | 0.921216 |
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Target: 5'- gGGCGGCaAGGUgcugcagCCCCG--UGCCGg -3' miRNA: 3'- aUCGUCG-UCUAaaa----GGGGCuuGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 84958 | 0.67 | 0.915297 |
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Target: 5'- cAGCGGCGGcauaaCCUCGuuGGCGCCGu -3' miRNA: 3'- aUCGUCGUCuaaaaGGGGC--UUGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 122239 | 0.67 | 0.915297 |
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Target: 5'- cUAGCAGCAGAcaagcgUCagCCGucuCGCCGc -3' miRNA: 3'- -AUCGUCGUCUaaa---AGg-GGCuu-GCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 21920 | 0.67 | 0.902691 |
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Target: 5'- aAGUAGCGcua---CCUCGAACGCCGc -3' miRNA: 3'- aUCGUCGUcuaaaaGGGGCUUGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 51328 | 0.69 | 0.858958 |
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Target: 5'- -cGCAGCGGGgucgUCCgCCGccGCGCCu -3' miRNA: 3'- auCGUCGUCUaaa-AGG-GGCu-UGCGGc -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 57606 | 0.69 | 0.850859 |
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Target: 5'- aUAGCAGCAGcg--UUUCCGAAUGCa- -3' miRNA: 3'- -AUCGUCGUCuaaaAGGGGCUUGCGgc -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 40899 | 0.7 | 0.779048 |
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Target: 5'- -uGCGGCAacuGUUUgugCuCCCGGACGCCGc -3' miRNA: 3'- auCGUCGUc--UAAAa--G-GGGCUUGCGGC- -5' |
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| 22885 | 3' | -53.3 | NC_005137.2 | + | 66323 | 1.07 | 0.005351 |
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Target: 5'- cUAGCAGCAGAUUUUCCCCGAACGCCGu -3' miRNA: 3'- -AUCGUCGUCUAAAAGGGGCUUGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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