Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22886 | 3' | -53.6 | NC_005137.2 | + | 35814 | 0.68 | 0.82751 |
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Target: 5'- cGCGUU-GCAAucuGcgUGCGUAGUGCAc -3' miRNA: 3'- aCGCAAgCGUU---CuaACGCGUCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 34958 | 0.68 | 0.853199 |
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Target: 5'- aGCGUUCGUcgcuAAG-UUGgGCGGCGguGg -3' miRNA: 3'- aCGCAAGCG----UUCuAACgCGUCGCguU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 33060 | 0.76 | 0.411058 |
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Target: 5'- cGCGUUCuaGAGAUUGUGCAGCa--- -3' miRNA: 3'- aCGCAAGcgUUCUAACGCGUCGcguu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 32419 | 0.68 | 0.861329 |
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Target: 5'- gGCGcUUUGCGAGG-UGCGCAacaGUGCGu -3' miRNA: 3'- aCGC-AAGCGUUCUaACGCGU---CGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 25684 | 0.66 | 0.929156 |
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Target: 5'- gGCGUUUGCAgacaagguGGAUUGggaCGCGGUGUc- -3' miRNA: 3'- aCGCAAGCGU--------UCUAAC---GCGUCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 22829 | 0.69 | 0.780895 |
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Target: 5'- cGCGUUgccaaaCGCGAGGgcggGCGC-GCGCAu -3' miRNA: 3'- aCGCAA------GCGUUCUaa--CGCGuCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 18580 | 0.7 | 0.761145 |
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Target: 5'- cGcCGUuucaaUUGCGAGAUUGCccGCAGCGUGAc -3' miRNA: 3'- aC-GCA-----AGCGUUCUAACG--CGUCGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 11765 | 0.68 | 0.844847 |
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Target: 5'- aGCGUUUauaaagGCuGGAagUGCGCAGuCGCAAg -3' miRNA: 3'- aCGCAAG------CGuUCUa-ACGCGUC-GCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 5250 | 0.68 | 0.861329 |
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Target: 5'- cGCGc-CGCucGAaacGCGCGGCGCAAg -3' miRNA: 3'- aCGCaaGCGuuCUaa-CGCGUCGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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