Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22886 | 3' | -53.6 | NC_005137.2 | + | 76635 | 0.69 | 0.818543 |
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Target: 5'- cGCGcuagcUUCGCu---UUGCGCGGCGCu- -3' miRNA: 3'- aCGC-----AAGCGuucuAACGCGUCGCGuu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 35814 | 0.68 | 0.82751 |
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Target: 5'- cGCGUU-GCAAucuGcgUGCGUAGUGCAc -3' miRNA: 3'- aCGCAAgCGUU---CuaACGCGUCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 72094 | 0.68 | 0.844847 |
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Target: 5'- cGCGUUucuggcgaacCGCAAGAUUGUGagcguuGUGCAAa -3' miRNA: 3'- aCGCAA----------GCGUUCUAACGCgu----CGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 34958 | 0.68 | 0.853199 |
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Target: 5'- aGCGUUCGUcgcuAAG-UUGgGCGGCGguGg -3' miRNA: 3'- aCGCAAGCG----UUCuAACgCGUCGCguU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 32419 | 0.68 | 0.861329 |
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Target: 5'- gGCGcUUUGCGAGG-UGCGCAacaGUGCGu -3' miRNA: 3'- aCGC-AAGCGUUCUaACGCGU---CGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 58656 | 0.66 | 0.911503 |
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Target: 5'- aGCGUgcaCGC-GGAgcgGCGCAgcucGCGCAAu -3' miRNA: 3'- aCGCAa--GCGuUCUaa-CGCGU----CGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 74753 | 0.66 | 0.917651 |
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Target: 5'- -cCGUU-GCAAcAUUGCGCGGUGCGg -3' miRNA: 3'- acGCAAgCGUUcUAACGCGUCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 75584 | 0.66 | 0.923536 |
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Target: 5'- gGCGgaCGCGGGcg-GCGCcguGCGCGu -3' miRNA: 3'- aCGCaaGCGUUCuaaCGCGu--CGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 63692 | 1.06 | 0.004749 |
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Target: 5'- uUGCGUUCGCAAGAUUGCGCAGCGCAAc -3' miRNA: 3'- -ACGCAAGCGUUCUAACGCGUCGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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