Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22886 | 3' | -53.6 | NC_005137.2 | + | 61076 | 0.69 | 0.771088 |
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Target: 5'- cGCGUauUCGUuuguggccGAcGGUUGCGUGGCGCAu -3' miRNA: 3'- aCGCA--AGCG--------UU-CUAACGCGUCGCGUu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 18580 | 0.7 | 0.761145 |
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Target: 5'- cGcCGUuucaaUUGCGAGAUUGCccGCAGCGUGAc -3' miRNA: 3'- aC-GCA-----AGCGUUCUAACG--CGUCGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 102847 | 0.7 | 0.751074 |
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Target: 5'- uUGCGUguaCGCuaacAAGAUUGCGCAaaGCGAg -3' miRNA: 3'- -ACGCAa--GCG----UUCUAACGCGUcgCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 98755 | 0.7 | 0.720213 |
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Target: 5'- uUGUGUUuggcgcccuaagUGCAGGGUUGCuaGGCGCGGa -3' miRNA: 3'- -ACGCAA------------GCGUUCUAACGcgUCGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 51740 | 0.71 | 0.677943 |
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Target: 5'- aUGCGUUaCGcCAAGAUUGUGCuGuUGCAAg -3' miRNA: 3'- -ACGCAA-GC-GUUCUAACGCGuC-GCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 83765 | 0.71 | 0.667246 |
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Target: 5'- uUGCGUUUGUAcGAccGCaGCGGCGCAGu -3' miRNA: 3'- -ACGCAAGCGUuCUaaCG-CGUCGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 67299 | 0.72 | 0.635004 |
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Target: 5'- aGCGUUUGUcucuGUUGCGCuGCGCAAu -3' miRNA: 3'- aCGCAAGCGuuc-UAACGCGuCGCGUU- -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 33060 | 0.76 | 0.411058 |
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Target: 5'- cGCGUUCuaGAGAUUGUGCAGCa--- -3' miRNA: 3'- aCGCAAGcgUUCUAACGCGUCGcguu -5' |
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| 22886 | 3' | -53.6 | NC_005137.2 | + | 63692 | 1.06 | 0.004749 |
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Target: 5'- uUGCGUUCGCAAGAUUGCGCAGCGCAAc -3' miRNA: 3'- -ACGCAAGCGUUCUAACGCGUCGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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