Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22889 | 3' | -56.6 | NC_005137.2 | + | 98019 | 0.68 | 0.715563 |
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Target: 5'- -uGCAaacauGugCGCGCACUUGgucagCGCCGGGu -3' miRNA: 3'- ccCGU-----CugGCGCGUGAAUa----GCGGCUU- -5' |
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| 22889 | 3' | -56.6 | NC_005137.2 | + | 102925 | 0.72 | 0.482034 |
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Target: 5'- cGGCAGGCCGCGCuGCgUGUacaGCCGu- -3' miRNA: 3'- cCCGUCUGGCGCG-UGaAUAg--CGGCuu -5' |
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| 22889 | 3' | -56.6 | NC_005137.2 | + | 110623 | 0.66 | 0.819815 |
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Target: 5'- uGGUgacGGCCGUGCACaaacgCGCCGAc -3' miRNA: 3'- cCCGu--CUGGCGCGUGaaua-GCGGCUu -5' |
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| 22889 | 3' | -56.6 | NC_005137.2 | + | 110723 | 0.66 | 0.836846 |
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Target: 5'- -cGguGugCGCGCagagcGCUUGUgCGCCGGg -3' miRNA: 3'- ccCguCugGCGCG-----UGAAUA-GCGGCUu -5' |
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| 22889 | 3' | -56.6 | NC_005137.2 | + | 114797 | 0.69 | 0.674798 |
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Target: 5'- uGGGCGGcUUGCGCACgucGUCGUCGu- -3' miRNA: 3'- -CCCGUCuGGCGCGUGaa-UAGCGGCuu -5' |
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| 22889 | 3' | -56.6 | NC_005137.2 | + | 129319 | 0.69 | 0.67891 |
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Target: 5'- cGGGacuaAGAUUGCGCGCguugcugaccgCGCCGAAa -3' miRNA: 3'- -CCCg---UCUGGCGCGUGaaua-------GCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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