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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22893 | 5' | -50.2 | NC_005137.2 | + | 93184 | 0.66 | 0.990758 |
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Target: 5'- -cGUCGG-UUGUGGCGUGCAGUCGa- -3' miRNA: 3'- uuCAGUUaAACACUGCGCGUUGGCca -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 125353 | 0.66 | 0.989404 |
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Target: 5'- aAGGUCAAUUUGUuuacGCGCGCAugUa-- -3' miRNA: 3'- -UUCAGUUAAACAc---UGCGCGUugGcca -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 81580 | 0.66 | 0.987899 |
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Target: 5'- cGGGUCAAaucuUGACGCGUGACgCGGc -3' miRNA: 3'- -UUCAGUUaaacACUGCGCGUUG-GCCa -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 28717 | 0.68 | 0.969275 |
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Target: 5'- uGGUgCAGUUUGaaaGGCGCGCAuuuGCUGGa -3' miRNA: 3'- uUCA-GUUAAACa--CUGCGCGU---UGGCCa -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 119615 | 0.68 | 0.969275 |
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Target: 5'- -uGcCAAUUUG-GcCGCGCAACUGGa -3' miRNA: 3'- uuCaGUUAAACaCuGCGCGUUGGCCa -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 123059 | 0.7 | 0.912877 |
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Target: 5'- cGGUCGAg--GUcgcGGCGCGCAGCCuuGGUg -3' miRNA: 3'- uUCAGUUaaaCA---CUGCGCGUUGG--CCA- -5' |
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| 22893 | 5' | -50.2 | NC_005137.2 | + | 43065 | 1.05 | 0.014379 |
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Target: 5'- uAAGUCAAUUUGUGACGCGCAACCGGUc -3' miRNA: 3'- -UUCAGUUAAACACUGCGCGUUGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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