Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22897 | 5' | -54 | NC_005137.2 | + | 18568 | 0.66 | 0.946111 |
Target: 5'- cGCCGCGuc-CAACGGCCgacuccaaCGCCGu- -3' miRNA: 3'- -CGGUGUuacGUUGCCGGaa------GCGGUcc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 55551 | 0.66 | 0.93158 |
Target: 5'- gGCCAaCAAUGCcACGGCCacgugcaagUUgGCCGc- -3' miRNA: 3'- -CGGU-GUUACGuUGCCGG---------AAgCGGUcc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 98849 | 0.67 | 0.926239 |
Target: 5'- aCCGCAuacAUGCuGACGGCUaUUGUguGGg -3' miRNA: 3'- cGGUGU---UACG-UUGCCGGaAGCGguCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 12819 | 0.67 | 0.926239 |
Target: 5'- cCCACAGcGcCAACGGCgucaaaguUUUCGgCCAGGc -3' miRNA: 3'- cGGUGUUaC-GUUGCCG--------GAAGC-GGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 48893 | 0.67 | 0.926239 |
Target: 5'- cGCCGC---GCuGCGG-CUUCGCCugAGGg -3' miRNA: 3'- -CGGUGuuaCGuUGCCgGAAGCGG--UCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 96419 | 0.67 | 0.920649 |
Target: 5'- cCCGCGcgGCGACGcGCCcgccgcguugcgUUUGCgCGGGu -3' miRNA: 3'- cGGUGUuaCGUUGC-CGG------------AAGCG-GUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 34582 | 0.67 | 0.920649 |
Target: 5'- -aCGCAAcGCGGCGGgCgcgUCGCCGcgcGGg -3' miRNA: 3'- cgGUGUUaCGUUGCCgGa--AGCGGU---CC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 21628 | 0.67 | 0.908722 |
Target: 5'- gGCUGCAGUGUGACGGUgg-CGaCGGGa -3' miRNA: 3'- -CGGUGUUACGUUGCCGgaaGCgGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 812 | 0.67 | 0.908722 |
Target: 5'- cGCUACAGgugGCGGCGGCacgUUUacaCCAGGu -3' miRNA: 3'- -CGGUGUUa--CGUUGCCGg--AAGc--GGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 28072 | 0.67 | 0.894468 |
Target: 5'- cGCCAgAcgGCuguacacgcagcGCGGCCU--GCCGGGu -3' miRNA: 3'- -CGGUgUuaCGu-----------UGCCGGAagCGGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 19495 | 0.68 | 0.881942 |
Target: 5'- aGCCGCucUGCGuccacuucuucaACGGaaaCggCGCCAGGc -3' miRNA: 3'- -CGGUGuuACGU------------UGCCg--GaaGCGGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 122692 | 0.68 | 0.881942 |
Target: 5'- aGCC-CGGcucGUAACGGCCgUUGCCAaGGu -3' miRNA: 3'- -CGGuGUUa--CGUUGCCGGaAGCGGU-CC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 13438 | 0.69 | 0.851469 |
Target: 5'- aGCCGCAccgGCGGC-GCaCUUUGCCGGu -3' miRNA: 3'- -CGGUGUua-CGUUGcCG-GAAGCGGUCc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 129004 | 0.69 | 0.817685 |
Target: 5'- cGCCGCAgcGUGCAcgagcugugcacGCGGUCaaCGaCCGGGu -3' miRNA: 3'- -CGGUGU--UACGU------------UGCCGGaaGC-GGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 34996 | 0.69 | 0.817685 |
Target: 5'- gGCgACGG-GCAACGGCCUgcaUGCCGa- -3' miRNA: 3'- -CGgUGUUaCGUUGCCGGAa--GCGGUcc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 103595 | 0.69 | 0.816802 |
Target: 5'- uGUUACAGcgcUGCAGCGGCCgcuacuuUUgGCCGGa -3' miRNA: 3'- -CGGUGUU---ACGUUGCCGG-------AAgCGGUCc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 93673 | 0.7 | 0.761922 |
Target: 5'- gGCCGCGuagaugcGCAACaGGCCcagCGCCGGa -3' miRNA: 3'- -CGGUGUua-----CGUUG-CCGGaa-GCGGUCc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 31806 | 0.71 | 0.742271 |
Target: 5'- cGCaGCGAUGCGGCGGCCcaaUGCCGc- -3' miRNA: 3'- -CGgUGUUACGUUGCCGGaa-GCGGUcc -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 8300 | 0.71 | 0.73229 |
Target: 5'- aGCaCGCGuaaccuugGCAACGGCCguuacgaGCCGGGc -3' miRNA: 3'- -CG-GUGUua------CGUUGCCGGaag----CGGUCC- -5' |
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22897 | 5' | -54 | NC_005137.2 | + | 127530 | 0.72 | 0.691558 |
Target: 5'- cGCCACAAUGU--CGGCCg-CGCUgAGGu -3' miRNA: 3'- -CGGUGUUACGuuGCCGGaaGCGG-UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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