Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
229 | 5' | -45.8 | AC_000008.1 | + | 8402 | 0.66 | 0.989962 |
Target: 5'- gGCGAC-CGcACCCUgugacGAAAGccGCCCg -3' miRNA: 3'- -CGCUGaGU-UGGGAac---CUUUUauUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 7127 | 0.66 | 0.984556 |
Target: 5'- cGCGGuCUUuccaguACUCUUGGAucggaAACCCg -3' miRNA: 3'- -CGCU-GAGu-----UGGGAACCUuuua-UUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 2874 | 0.66 | 0.984556 |
Target: 5'- uUGGCUCGACaggaaaCCgugUGGAAuAUAACaCCu -3' miRNA: 3'- cGCUGAGUUG------GGa--ACCUUuUAUUG-GG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 32739 | 0.66 | 0.984556 |
Target: 5'- cUGGC-CAGgCCUUGcGAGAGUGGCUg -3' miRNA: 3'- cGCUGaGUUgGGAAC-CUUUUAUUGGg -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 29963 | 0.67 | 0.979879 |
Target: 5'- gGCGACaugugucuUCAcACCC-UGGAgcGAGUgGACCCc -3' miRNA: 3'- -CGCUG--------AGU-UGGGaACCU--UUUA-UUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 11319 | 0.68 | 0.966004 |
Target: 5'- cGCGGCagaaccuguuucgCGACCgCgagGGAGAggAGCCCg -3' miRNA: 3'- -CGCUGa------------GUUGG-Gaa-CCUUUuaUUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 19067 | 0.68 | 0.950472 |
Target: 5'- cGCGGUUC-ACCCUagcugUGGguGAUAACCg -3' miRNA: 3'- -CGCUGAGuUGGGA-----ACCuuUUAUUGGg -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 33959 | 0.7 | 0.900955 |
Target: 5'- aUGAC-CuuCUCUUGGAAAagcgccugGUGACCCa -3' miRNA: 3'- cGCUGaGuuGGGAACCUUU--------UAUUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 30582 | 0.71 | 0.884493 |
Target: 5'- cGCuGACgagGACCCUgcgcccgUGGAAAgacAUGACCCu -3' miRNA: 3'- -CG-CUGag-UUGGGA-------ACCUUU---UAUUGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 5474 | 0.71 | 0.869292 |
Target: 5'- cGCGGCgUGGCCCUUGGcgcgcagcuuGCCCu -3' miRNA: 3'- -CGCUGaGUUGGGAACCuuuuau----UGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 17065 | 0.71 | 0.868419 |
Target: 5'- aGCGACUgGAagaugUCUUGGAaaAAAUGACCg -3' miRNA: 3'- -CGCUGAgUUg----GGAACCU--UUUAUUGGg -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 35305 | 0.71 | 0.859528 |
Target: 5'- aGCGACcgaaaUAGCCCg-GGGGAAUAcauACCCg -3' miRNA: 3'- -CGCUGa----GUUGGGaaCCUUUUAU---UGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 27970 | 0.73 | 0.789953 |
Target: 5'- cCGGCUUAccGCCCagGGAGAGcuuGCCCg -3' miRNA: 3'- cGCUGAGU--UGGGaaCCUUUUau-UGGG- -5' |
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229 | 5' | -45.8 | AC_000008.1 | + | 24991 | 1.17 | 0.00208 |
Target: 5'- cGCGACUCAACCCUUGGAAAAUAACCCu -3' miRNA: 3'- -CGCUGAGUUGGGAACCUUUUAUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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