Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22900 | 5' | -52.2 | NC_005137.2 | + | 751 | 0.66 | 0.97384 |
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Target: 5'- cAAGCG--GCGGUGGCG-GCGGCAc-- -3' miRNA: 3'- -UUCGCacUGCCACUGCaUGCUGUacu -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 68674 | 0.66 | 0.967909 |
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Target: 5'- -cGCGUGGCGGaUGacagcauaauaaGCGUGCGcaaaagcaauuuGCGUGAc -3' miRNA: 3'- uuCGCACUGCC-AC------------UGCAUGC------------UGUACU- -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 26530 | 0.67 | 0.961082 |
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Target: 5'- aAAGUGUGGCGc--GCGUACGGCAa-- -3' miRNA: 3'- -UUCGCACUGCcacUGCAUGCUGUacu -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 105847 | 0.67 | 0.957317 |
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Target: 5'- -cGCGUGcuccagacACGGaGGCGUGCGAguUGGc -3' miRNA: 3'- uuCGCAC--------UGCCaCUGCAUGCUguACU- -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 110431 | 0.67 | 0.957317 |
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Target: 5'- cGGGCGcGACGGUGAgCGUGC-ACAc-- -3' miRNA: 3'- -UUCGCaCUGCCACU-GCAUGcUGUacu -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 72672 | 0.67 | 0.949058 |
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Target: 5'- uGAGCGaUGACGcUGuuAgGUACGAUAUGAg -3' miRNA: 3'- -UUCGC-ACUGCcAC--UgCAUGCUGUACU- -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 102885 | 0.68 | 0.945018 |
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Target: 5'- uGGGCuGUGACGGggguuugcugcacacGGCGUACGAggccguCAUGAa -3' miRNA: 3'- -UUCG-CACUGCCa--------------CUGCAUGCU------GUACU- -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 41160 | 0.68 | 0.944556 |
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Target: 5'- uGGGCGcagacGGCGGUGugGUA-GGCGUGc -3' miRNA: 3'- -UUCGCa----CUGCCACugCAUgCUGUACu -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 21633 | 0.72 | 0.764719 |
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Target: 5'- cAGUGUGACGGUGGCG-ACGGgAg-- -3' miRNA: 3'- uUCGCACUGCCACUGCaUGCUgUacu -5' |
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| 22900 | 5' | -52.2 | NC_005137.2 | + | 32664 | 1.07 | 0.007994 |
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Target: 5'- cAAGCGUGACGGUGACGUACGACAUGAc -3' miRNA: 3'- -UUCGCACUGCCACUGCAUGCUGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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