Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22901 | 5' | -50.6 | NC_005137.2 | + | 40374 | 0.66 | 0.993676 |
Target: 5'- aCCACCAUGAAuCGCAuucuuGUAAAaUGGGu -3' miRNA: 3'- gGGUGGUACUUcGCGU-----CGUUUaGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 46634 | 0.66 | 0.993676 |
Target: 5'- aCCGCCAUGGuGGacaGCGGCGcua-AGGg -3' miRNA: 3'- gGGUGGUACU-UCg--CGUCGUuuagUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 68763 | 0.66 | 0.993676 |
Target: 5'- uCCUACCAcaaUGucuacaauGGUGCAGCGGA-CAGa -3' miRNA: 3'- -GGGUGGU---ACu-------UCGCGUCGUUUaGUCc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 40539 | 0.66 | 0.992688 |
Target: 5'- aCCACaaugGAAGCGCAGCGu------ -3' miRNA: 3'- gGGUGgua-CUUCGCGUCGUuuagucc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 22398 | 0.66 | 0.992688 |
Target: 5'- gCCACCAgguugugcuUGAAGCGCaccacguaaaAGCAca-CGGGc -3' miRNA: 3'- gGGUGGU---------ACUUCGCG----------UCGUuuaGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 96332 | 0.66 | 0.992583 |
Target: 5'- cCCCACgGcgcuugcUGguGCgGUGGCGGGUCGGGu -3' miRNA: 3'- -GGGUGgU-------ACuuCG-CGUCGUUUAGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 16891 | 0.66 | 0.991581 |
Target: 5'- uUCAUCcgGAAGCGguGCAGAcgcacgcggcgcUCGGc -3' miRNA: 3'- gGGUGGuaCUUCGCguCGUUU------------AGUCc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 16559 | 0.66 | 0.991581 |
Target: 5'- aCCCACCucauUGGAGCGaAGUGAguGUUAuGGa -3' miRNA: 3'- -GGGUGGu---ACUUCGCgUCGUU--UAGU-CC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 50224 | 0.66 | 0.988971 |
Target: 5'- --aGCCAaacucGCGCAGCAGcUCGGGg -3' miRNA: 3'- gggUGGUacuu-CGCGUCGUUuAGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 61443 | 0.66 | 0.988971 |
Target: 5'- gUuuGCCGUGucGCGUGGCAAGUUg-- -3' miRNA: 3'- -GggUGGUACuuCGCGUCGUUUAGucc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 123054 | 0.66 | 0.988971 |
Target: 5'- gCCGCCGgucGAGGuCGCGGCGcgcagccuuGGUgAGGu -3' miRNA: 3'- gGGUGGUa--CUUC-GCGUCGU---------UUAgUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 118231 | 0.66 | 0.987449 |
Target: 5'- cCCCAaCAUuuGGCGCAGUAgcucGAUCGGc -3' miRNA: 3'- -GGGUgGUAcuUCGCGUCGU----UUAGUCc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 124967 | 0.67 | 0.983925 |
Target: 5'- -gCGCCGgugaUGggGCGCuGCGcugcguuggaAAUCGGGc -3' miRNA: 3'- ggGUGGU----ACuuCGCGuCGU----------UUAGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 32464 | 0.67 | 0.981904 |
Target: 5'- gCCAaaauaCAUGgcGUGCAGCAucuUCAGc -3' miRNA: 3'- gGGUg----GUACuuCGCGUCGUuu-AGUCc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 9330 | 0.67 | 0.981904 |
Target: 5'- uUUAUCAUGGAucGCGUAGCAGAccaaauuuacUCGGGc -3' miRNA: 3'- gGGUGGUACUU--CGCGUCGUUU----------AGUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 79587 | 0.67 | 0.979698 |
Target: 5'- gCCGCCGUGcgauAGCGUcGCAuAUCAa- -3' miRNA: 3'- gGGUGGUACu---UCGCGuCGUuUAGUcc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 110023 | 0.68 | 0.971882 |
Target: 5'- aCCCGuuugCGUGcAAGCGCGGCAAAacgcgcgccgCAGGc -3' miRNA: 3'- -GGGUg---GUAC-UUCGCGUCGUUUa---------GUCC- -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 63006 | 0.7 | 0.935987 |
Target: 5'- aCCCAUgGUGcGGCGCcGGCGuGUCGGc -3' miRNA: 3'- -GGGUGgUACuUCGCG-UCGUuUAGUCc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 36616 | 0.71 | 0.900583 |
Target: 5'- gUCCACCugcggguUGggGCGCAcCGAGUCAa- -3' miRNA: 3'- -GGGUGGu------ACuuCGCGUcGUUUAGUcc -5' |
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22901 | 5' | -50.6 | NC_005137.2 | + | 98008 | 0.72 | 0.839445 |
Target: 5'- gCCCGCCAagUGGccAGCGU-GCAcGUCGGGc -3' miRNA: 3'- -GGGUGGU--ACU--UCGCGuCGUuUAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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