miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22902 3' -57.2 NC_005137.2 + 5163 0.66 0.812564
Target:  5'- uGGCuAGGgGUGGCuGGCUGaagcuggcUGACuGGUa -3'
miRNA:   3'- -CUG-UCCgCACCGuCCGAU--------ACUG-CCAc -5'
22902 3' -57.2 NC_005137.2 + 8634 0.66 0.803651
Target:  5'- cGGCGGGCGUgGGCGuGCUAUuGGcCGGa- -3'
miRNA:   3'- -CUGUCCGCA-CCGUcCGAUA-CU-GCCac -5'
22902 3' -57.2 NC_005137.2 + 21617 0.67 0.766497
Target:  5'- cGAC-GGUGU--CAGGCUgcaguGUGACGGUGg -3'
miRNA:   3'- -CUGuCCGCAccGUCCGA-----UACUGCCAC- -5'
22902 3' -57.2 NC_005137.2 + 72010 0.68 0.724393
Target:  5'- uGACAGGCGgcucauuuacaacuUGGUGGGC----ACGGUGg -3'
miRNA:   3'- -CUGUCCGC--------------ACCGUCCGauacUGCCAC- -5'
22902 3' -57.2 NC_005137.2 + 78433 0.68 0.695103
Target:  5'- uGGCugAGGCgGUGGCuguugagaugguGGCUGaGGCGGUGg -3'
miRNA:   3'- -CUG--UCCG-CACCGu-----------CCGAUaCUGCCAC- -5'
22902 3' -57.2 NC_005137.2 + 116822 0.73 0.426267
Target:  5'- uGGCAGGCGcGGCAGGCUuuguguuugagGACGcGUa -3'
miRNA:   3'- -CUGUCCGCaCCGUCCGAua---------CUGC-CAc -5'
22902 3' -57.2 NC_005137.2 + 30020 1.08 0.001897
Target:  5'- gGACAGGCGUGGCAGGCUAUGACGGUGa -3'
miRNA:   3'- -CUGUCCGCACCGUCCGAUACUGCCAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.