Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22903 | 3' | -58.9 | NC_005137.2 | + | 88585 | 0.66 | 0.781763 |
Target: 5'- cGGCGGACAcAUnACguGUCcCGGCGGg -3' miRNA: 3'- -CCGCCUGUcUGgUGguCGGaGUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 64661 | 0.66 | 0.763231 |
Target: 5'- uGGCGGGCAauGACCcggACaCGGCgUUCGGgGAa -3' miRNA: 3'- -CCGCCUGU--CUGG---UG-GUCG-GAGUCgCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 81291 | 0.66 | 0.763231 |
Target: 5'- aGCGGAC-GAaacaccuaCACCGGCCaagCGGCGc -3' miRNA: 3'- cCGCCUGuCUg-------GUGGUCGGa--GUCGCu -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 123540 | 0.66 | 0.752839 |
Target: 5'- cGGUGGGCGGcuauugcACCACaaacagcguGCCUC-GCGAu -3' miRNA: 3'- -CCGCCUGUC-------UGGUGgu-------CGGAGuCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 51294 | 0.66 | 0.734601 |
Target: 5'- cGGCGGGCagcggcauaacgAGugCGCCAaGUaacgCAGCGGg -3' miRNA: 3'- -CCGCCUG------------UCugGUGGU-CGga--GUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 130618 | 0.67 | 0.675232 |
Target: 5'- cGCGGACAcgcgcgccGACaaaGCCAGCaaaAGCGAg -3' miRNA: 3'- cCGCCUGU--------CUGg--UGGUCGgagUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 32208 | 0.68 | 0.655074 |
Target: 5'- cGGCGGcguuugGCAGACgACaUGGCCUUGGCa- -3' miRNA: 3'- -CCGCC------UGUCUGgUG-GUCGGAGUCGcu -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 130571 | 0.68 | 0.655074 |
Target: 5'- cGGuCGGACGuuGCCACCAGCgaaucaacCAGCGGa -3' miRNA: 3'- -CC-GCCUGUc-UGGUGGUCGga------GUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 55770 | 0.68 | 0.655074 |
Target: 5'- cGCGGuCAcGGCCAucauuUCGGCCagCAGCGAa -3' miRNA: 3'- cCGCCuGU-CUGGU-----GGUCGGa-GUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 35492 | 0.68 | 0.655074 |
Target: 5'- aGCGca-AGucGCCACCGGCCacgUCAGCGAu -3' miRNA: 3'- cCGCcugUC--UGGUGGUCGG---AGUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 99310 | 0.69 | 0.554421 |
Target: 5'- uGGUGGGCGGGCCGCUgccaucaaggauGGCaaaCAGCGc -3' miRNA: 3'- -CCGCCUGUCUGGUGG------------UCGga-GUCGCu -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 88492 | 0.7 | 0.519123 |
Target: 5'- gGGCGGGCgAGcgauuagcgcgcuauACCGCCGGUCUUuuuGCGGg -3' miRNA: 3'- -CCGCCUG-UC---------------UGGUGGUCGGAGu--CGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 78710 | 0.72 | 0.39732 |
Target: 5'- gGGUGG-CAGAacggCugCGGCUUCGGCGAu -3' miRNA: 3'- -CCGCCuGUCUg---GugGUCGGAGUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 16901 | 0.77 | 0.203869 |
Target: 5'- aGCGGuGCAGACgCACgCGGCgCUCGGCGAa -3' miRNA: 3'- cCGCC-UGUCUG-GUG-GUCG-GAGUCGCU- -5' |
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22903 | 3' | -58.9 | NC_005137.2 | + | 27451 | 1.1 | 0.001157 |
Target: 5'- uGGCGGACAGACCACCAGCCUCAGCGAc -3' miRNA: 3'- -CCGCCUGUCUGGUGGUCGGAGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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