Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22904 | 5' | -55.9 | NC_005137.2 | + | 12880 | 0.66 | 0.908335 |
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Target: 5'- cGGGCGCGgGGcgaaacAGGUg--GCCGAacacgAGCg -3' miRNA: 3'- cCUCGUGCgCC------UCCAagaCGGCU-----UCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 70275 | 0.66 | 0.908335 |
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Target: 5'- aGGuGUugGCGGAGGcgUCgGUgaAGGCg -3' miRNA: 3'- -CCuCGugCGCCUCCa-AGaCGgcUUCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 13021 | 0.66 | 0.902096 |
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Target: 5'- cGGGUugGUGGcGGGUgagCUGCUGuuGUu -3' miRNA: 3'- cCUCGugCGCC-UCCAa--GACGGCuuCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 111170 | 0.67 | 0.844058 |
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Target: 5'- cGGGCG-GCGGAGGcuUUgUGUCGcGGCg -3' miRNA: 3'- cCUCGUgCGCCUCC--AAgACGGCuUCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 70323 | 0.68 | 0.818926 |
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Target: 5'- aGGuGUugGCGGAGGcgUC-GgCGAAGg -3' miRNA: 3'- -CCuCGugCGCCUCCa-AGaCgGCUUCg -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 100050 | 0.68 | 0.810195 |
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Target: 5'- -cGGCGCGCGGcGcGUUCagcaugcuuugUGCCGcGGCg -3' miRNA: 3'- ccUCGUGCGCCuC-CAAG-----------ACGGCuUCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 78367 | 0.69 | 0.77373 |
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Target: 5'- aGGAGguUgcuGCGGuGGUg--GCUGAGGCg -3' miRNA: 3'- -CCUCguG---CGCCuCCAagaCGGCUUCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 31007 | 0.71 | 0.613376 |
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Target: 5'- uGGAGUugGUGGcGGUUggugcgugcgCUGCUGgcGCa -3' miRNA: 3'- -CCUCGugCGCCuCCAA----------GACGGCuuCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 17479 | 0.73 | 0.502834 |
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Target: 5'- uGGGGCugGUGGuGGUgCaGCCGuuGCa -3' miRNA: 3'- -CCUCGugCGCCuCCAaGaCGGCuuCG- -5' |
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| 22904 | 5' | -55.9 | NC_005137.2 | + | 25173 | 1.14 | 0.001195 |
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Target: 5'- uGGAGCACGCGGAGGUUCUGCCGAAGCg -3' miRNA: 3'- -CCUCGUGCGCCUCCAAGACGGCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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