Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22908 | 3' | -53.9 | NC_005137.2 | + | 474 | 0.72 | 0.65906 |
Target: 5'- -cCGCCGCGGCGGCUgcccAGCgugUUGuACa -3' miRNA: 3'- gaGUGGUGCCGCCGGuu--UCGa--AAC-UG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 769 | 0.68 | 0.860414 |
Target: 5'- -gCACguuUGGCGGCgGAGGCggUGGCa -3' miRNA: 3'- gaGUGgu-GCCGCCGgUUUCGaaACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 3546 | 0.67 | 0.91252 |
Target: 5'- -gCACCGguUGGCGGCUAaaaaGagcaagcguacuauuAGCUUUGACu -3' miRNA: 3'- gaGUGGU--GCCGCCGGU----U---------------UCGAAACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 15255 | 0.66 | 0.922007 |
Target: 5'- -aCGCCGCGGCGcGCaCGuuGCguugcaccUUGGCg -3' miRNA: 3'- gaGUGGUGCCGC-CG-GUuuCGa-------AACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 19075 | 1.1 | 0.003121 |
Target: 5'- gCUCACCACGGCGGCCAAAGCUUUGACu -3' miRNA: 3'- -GAGUGGUGCCGCCGGUUUCGAAACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 21586 | 0.68 | 0.868212 |
Target: 5'- --gGCCGCGcauaGGCCGcGGUUUUGACg -3' miRNA: 3'- gagUGGUGCcg--CCGGUuUCGAAACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 27078 | 0.69 | 0.827092 |
Target: 5'- uUCACgAccuuguuguguCGGUGGCCAAAGUg--GACg -3' miRNA: 3'- gAGUGgU-----------GCCGCCGGUUUCGaaaCUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 69195 | 0.67 | 0.897076 |
Target: 5'- -gCGCUuaacgaGGCGGCCAAacAGCUUagucUGGCu -3' miRNA: 3'- gaGUGGug----CCGCCGGUU--UCGAA----ACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 86186 | 0.67 | 0.903686 |
Target: 5'- -aCGCCGacaaGGCGGCCGAcGUgUUUGAa -3' miRNA: 3'- gaGUGGUg---CCGCCGGUUuCG-AAACUg -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 86955 | 0.66 | 0.939996 |
Target: 5'- gCUCAUCAacgcgucugaaaugcCGGCGGCCAaaAAGUUUaaGCa -3' miRNA: 3'- -GAGUGGU---------------GCCGCCGGU--UUCGAAacUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 97800 | 0.67 | 0.897076 |
Target: 5'- uCUCGCCGCGGUGcGCUcuucGAcGCgcaaUUGACc -3' miRNA: 3'- -GAGUGGUGCCGC-CGG----UUuCGa---AACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 100146 | 0.68 | 0.860414 |
Target: 5'- gCUCGCCcacguACgGGCGcGCCGGgucgucAGCUUUGAUu -3' miRNA: 3'- -GAGUGG-----UG-CCGC-CGGUU------UCGAAACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 107402 | 0.66 | 0.932946 |
Target: 5'- aUUGCgACGGCGGCCucaaagacugggAAAGCaccGACg -3' miRNA: 3'- gAGUGgUGCCGCCGG------------UUUCGaaaCUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 107963 | 0.66 | 0.938032 |
Target: 5'- -cCGCCACGGUGaacaccuCCAgcGAGCcgUUGACa -3' miRNA: 3'- gaGUGGUGCCGCc------GGU--UUCGa-AACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 111156 | 0.68 | 0.851583 |
Target: 5'- gUCACUggcguaaACgGGCGGCgGAGGCUUUGuguCg -3' miRNA: 3'- gAGUGG-------UG-CCGCCGgUUUCGAAACu--G- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 112616 | 0.72 | 0.637913 |
Target: 5'- uCUCGCCcaaaGCGGCGGCaguAGGCgucgUUGAa -3' miRNA: 3'- -GAGUGG----UGCCGCCGgu-UUCGa---AACUg -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 118169 | 0.7 | 0.761843 |
Target: 5'- --gGCUACGGCcuGGCCGAAaaCUUUGACg -3' miRNA: 3'- gagUGGUGCCG--CCGGUUUc-GAAACUG- -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 119779 | 0.67 | 0.897076 |
Target: 5'- gCUCACCAaacguguuuuauCGGCgacGGCCGGAGCggcgcccauUUUGAa -3' miRNA: 3'- -GAGUGGU------------GCCG---CCGGUUUCG---------AAACUg -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 120320 | 0.66 | 0.916154 |
Target: 5'- -aCGCCacgaaACGGUGGCCAAAGUa----- -3' miRNA: 3'- gaGUGG-----UGCCGCCGGUUUCGaaacug -5' |
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22908 | 3' | -53.9 | NC_005137.2 | + | 122070 | 0.67 | 0.897076 |
Target: 5'- gUguCCGgguCGGCGGCCGGGucaCUUUGACg -3' miRNA: 3'- gAguGGU---GCCGCCGGUUUc--GAAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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