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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22908 | 5' | -52.2 | NC_005137.2 | + | 111928 | 0.69 | 0.90892 |
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Target: 5'- -aGUCAAAGCUuugGCcgCCGUGGUGaGCg -3' miRNA: 3'- ggUAGUUUCGG---CGaaGGCAUCACcCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 102756 | 0.66 | 0.974865 |
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Target: 5'- -aAUCAAGGCaCGUgcggUgCGUGuUGGGCg -3' miRNA: 3'- ggUAGUUUCG-GCGa---AgGCAUcACCCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 91330 | 0.75 | 0.6477 |
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Target: 5'- gCCAaCuGAGCCGCUuguaUUCGUuuGUGGGCa -3' miRNA: 3'- -GGUaGuUUCGGCGA----AGGCAu-CACCCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 81440 | 0.68 | 0.950891 |
|
Target: 5'- aCCuguUCAAAGCaauGC-UCCG-AGUGGcGCa -3' miRNA: 3'- -GGu--AGUUUCGg--CGaAGGCaUCACC-CG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 58991 | 0.67 | 0.96595 |
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Target: 5'- cCCAcCAaguuguaaauGAGCCGCcugUCaaucUGGUGGGCg -3' miRNA: 3'- -GGUaGU----------UUCGGCGa--AGgc--AUCACCCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 36486 | 0.67 | 0.958893 |
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Target: 5'- gCCGUCAA--CCGUcacaacgauuuUUCCGUucacGGUGGGUu -3' miRNA: 3'- -GGUAGUUucGGCG-----------AAGGCA----UCACCCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 30845 | 0.72 | 0.80805 |
|
Target: 5'- -aAUCAAAGCugacgacccggCGCgcCCGUAcGUGGGCg -3' miRNA: 3'- ggUAGUUUCG-----------GCGaaGGCAU-CACCCG- -5' |
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| 22908 | 5' | -52.2 | NC_005137.2 | + | 19109 | 1.15 | 0.002458 |
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Target: 5'- gCCAUCAAAGCCGCUUCCGUAGUGGGCa -3' miRNA: 3'- -GGUAGUUUCGGCGAAGGCAUCACCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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