miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22910 5' -53.2 NC_005137.2 + 13457 1.04 0.00716
Target:  5'- uUUGCCGGUACACCAACAGGUUAGGAUg -3'
miRNA:   3'- -AACGGCCAUGUGGUUGUCCAAUCCUA- -5'
22910 5' -53.2 NC_005137.2 + 61735 0.71 0.725013
Target:  5'- cUGCUGGUcaaACGgCAACGGGaUAGGAa -3'
miRNA:   3'- aACGGCCA---UGUgGUUGUCCaAUCCUa -5'
22910 5' -53.2 NC_005137.2 + 71704 0.67 0.895016
Target:  5'- gUGCUGGUGcCGCCc---GGUUGGGAa -3'
miRNA:   3'- aACGGCCAU-GUGGuuguCCAAUCCUa -5'
22910 5' -53.2 NC_005137.2 + 96342 0.69 0.822901
Target:  5'- cUUGCUGGUGCGgUGGCGGGUcGGGu- -3'
miRNA:   3'- -AACGGCCAUGUgGUUGUCCAaUCCua -5'
22910 5' -53.2 NC_005137.2 + 98413 0.73 0.585949
Target:  5'- gUGCCGGUgauGCACCAACGGuuGUUGGcGGUu -3'
miRNA:   3'- aACGGCCA---UGUGGUUGUC--CAAUC-CUA- -5'
22910 5' -53.2 NC_005137.2 + 103810 0.67 0.895016
Target:  5'- gUUGCUGGUguGCGgCGGCGGGUUGGu-- -3'
miRNA:   3'- -AACGGCCA--UGUgGUUGUCCAAUCcua -5'
22910 5' -53.2 NC_005137.2 + 107355 0.68 0.839626
Target:  5'- -aGCCGGUuuuuauuACACCGGCaaGGGUgacAGGGUc -3'
miRNA:   3'- aaCGGCCA-------UGUGGUUG--UCCAa--UCCUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.