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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22910 | 5' | -53.2 | NC_005137.2 | + | 13457 | 1.04 | 0.00716 |
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Target: 5'- uUUGCCGGUACACCAACAGGUUAGGAUg -3' miRNA: 3'- -AACGGCCAUGUGGUUGUCCAAUCCUA- -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 61735 | 0.71 | 0.725013 |
|
Target: 5'- cUGCUGGUcaaACGgCAACGGGaUAGGAa -3' miRNA: 3'- aACGGCCA---UGUgGUUGUCCaAUCCUa -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 71704 | 0.67 | 0.895016 |
|
Target: 5'- gUGCUGGUGcCGCCc---GGUUGGGAa -3' miRNA: 3'- aACGGCCAU-GUGGuuguCCAAUCCUa -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 96342 | 0.69 | 0.822901 |
|
Target: 5'- cUUGCUGGUGCGgUGGCGGGUcGGGu- -3' miRNA: 3'- -AACGGCCAUGUgGUUGUCCAaUCCua -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 98413 | 0.73 | 0.585949 |
|
Target: 5'- gUGCCGGUgauGCACCAACGGuuGUUGGcGGUu -3' miRNA: 3'- aACGGCCA---UGUGGUUGUC--CAAUC-CUA- -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 103810 | 0.67 | 0.895016 |
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Target: 5'- gUUGCUGGUguGCGgCGGCGGGUUGGu-- -3' miRNA: 3'- -AACGGCCA--UGUgGUUGUCCAAUCcua -5' |
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| 22910 | 5' | -53.2 | NC_005137.2 | + | 107355 | 0.68 | 0.839626 |
|
Target: 5'- -aGCCGGUuuuuauuACACCGGCaaGGGUgacAGGGUc -3' miRNA: 3'- aaCGGCCA-------UGUGGUUG--UCCAa--UCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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