Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22912 | 3' | -54.5 | NC_005137.2 | + | 3325 | 0.67 | 0.875894 |
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Target: 5'- gUAGCCAaagugaUugACCUGUCGGGccgCGCg -3' miRNA: 3'- gAUUGGUg-----AugUGGGCGGCCCaa-GUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 1814 | 0.67 | 0.875894 |
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Target: 5'- -aAACUGCUuACGCCUGCggcaGGGUUcCACa -3' miRNA: 3'- gaUUGGUGA-UGUGGGCGg---CCCAA-GUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 35643 | 0.68 | 0.852832 |
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Target: 5'- -cAGCCAC-ACGCCCGUacUGGGauugCACg -3' miRNA: 3'- gaUUGGUGaUGUGGGCG--GCCCaa--GUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 48884 | 0.68 | 0.836405 |
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Target: 5'- -cAugUACUGCGCCgCGCUGcGGcUUCGCc -3' miRNA: 3'- gaUugGUGAUGUGG-GCGGC-CC-AAGUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 96410 | 0.68 | 0.825308 |
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Target: 5'- -cGACCGCUacccgcgcggcgacGCGCCCGCCGcguugcGUUUGCg -3' miRNA: 3'- gaUUGGUGA--------------UGUGGGCGGCc-----CAAGUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 92954 | 0.7 | 0.734083 |
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Target: 5'- --uACCACcucAgACCUGCUGGGUUCAa -3' miRNA: 3'- gauUGGUGa--UgUGGGCGGCCCAAGUg -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 16812 | 0.72 | 0.651925 |
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Target: 5'- uCUAACCccguaauuaACUGCAgCCGgugCGGGUUCACu -3' miRNA: 3'- -GAUUGG---------UGAUGUgGGCg--GCCCAAGUG- -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 5252 | 0.72 | 0.641485 |
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Target: 5'- --cGCCGCUcgaaacgcgcgGCGCaaGCCGGGUUCAa -3' miRNA: 3'- gauUGGUGA-----------UGUGggCGGCCCAAGUg -5' |
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| 22912 | 3' | -54.5 | NC_005137.2 | + | 8254 | 1.09 | 0.003119 |
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Target: 5'- gCUAACCACUACACCCGCCGGGUUCACu -3' miRNA: 3'- -GAUUGGUGAUGUGGGCGGCCCAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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