Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22913 | 3' | -47.9 | NC_005137.2 | + | 127101 | 0.82 | 0.45684 |
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Target: 5'- gAGUCAGCAUUaACCcGGUUUUACAAGu -3' miRNA: 3'- -UCAGUCGUAAcUGGaCCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 6794 | 0.92 | 0.137376 |
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Target: 5'- cGUCAGCGUUGACCUcGCUUUACGAGc -3' miRNA: 3'- uCAGUCGUAACUGGAcCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 127035 | 1.1 | 0.011646 |
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Target: 5'- gAGUCAGCAUUGACCUGGCUUUACAAGu -3' miRNA: 3'- -UCAGUCGUAACUGGACCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 125188 | 0.74 | 0.861958 |
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Target: 5'- gAGUUGGCGUUGAUCUGGCg--ACu-- -3' miRNA: 3'- -UCAGUCGUAACUGGACCGaaaUGuuc -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 22833 | 0.74 | 0.870083 |
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Target: 5'- cGUCAcgGUUGACCUcGCUUUACGAGu -3' miRNA: 3'- uCAGUcgUAACUGGAcCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 76950 | 0.72 | 0.931281 |
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Target: 5'- cGUCAGCGccGACCUcGCUUUuCAAGa -3' miRNA: 3'- uCAGUCGUaaCUGGAcCGAAAuGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 15887 | 0.72 | 0.941826 |
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Target: 5'- cGUCAuuGUUGACCUuGUUUUACAAGu -3' miRNA: 3'- uCAGUcgUAACUGGAcCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 126844 | 0.71 | 0.951261 |
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Target: 5'- -aUCAGCGcUGACUU-GCUUUACGAGu -3' miRNA: 3'- ucAGUCGUaACUGGAcCGAAAUGUUC- -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 113588 | 0.69 | 0.983186 |
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Target: 5'- cGUCAGCGUUGACCUuGUUUg----- -3' miRNA: 3'- uCAGUCGUAACUGGAcCGAAauguuc -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 79759 | 0.66 | 0.998913 |
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Target: 5'- cGUUAGCGUUGGCgUUGGCgguuugugaUUUGCGu- -3' miRNA: 3'- uCAGUCGUAACUG-GACCG---------AAAUGUuc -5' |
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| 22913 | 3' | -47.9 | NC_005137.2 | + | 86438 | 0.66 | 0.998913 |
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Target: 5'- aGGUauGCGUuauacaUGACCUuGCUUUACAAGc -3' miRNA: 3'- -UCAguCGUA------ACUGGAcCGAAAUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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