Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22913 | 5' | -50.2 | NC_005137.2 | + | 96691 | 0.66 | 0.987704 |
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Target: 5'- cGGC-CGUAcacaaaAAGCGGGuUCGGCGUUGAc -3' miRNA: 3'- -UUGaGCAU------UUCGUUC-AGUCGCGACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 5446 | 0.66 | 0.982022 |
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Target: 5'- aAACUCG---AGCGAGUCuAGCGCa--- -3' miRNA: 3'- -UUGAGCauuUCGUUCAG-UCGCGacua -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 100599 | 0.68 | 0.957507 |
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Target: 5'- uACUCGUGccugaAAGacgAGGUUGGCGCUGAc -3' miRNA: 3'- uUGAGCAU-----UUCg--UUCAGUCGCGACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 6510 | 0.68 | 0.944181 |
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Target: 5'- uACUCGUAAAGCGAgGUCaucaauGGCGCa--- -3' miRNA: 3'- uUGAGCAUUUCGUU-CAG------UCGCGacua -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 38332 | 0.71 | 0.874076 |
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Target: 5'- uACUCGUAGGGCGuaAG-CAGCGCguUGAc -3' miRNA: 3'- uUGAGCAUUUCGU--UCaGUCGCG--ACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 126876 | 0.72 | 0.822648 |
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Target: 5'- uACUUGUAAAGcCAGGUCAaUGCUGAc -3' miRNA: 3'- uUGAGCAUUUC-GUUCAGUcGCGACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 77107 | 0.74 | 0.711188 |
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Target: 5'- -uCUUGaAAAGCgAGGUCGGCGCUGAc -3' miRNA: 3'- uuGAGCaUUUCG-UUCAGUCGCGACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 6637 | 0.8 | 0.404703 |
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Target: 5'- uGCUCGUAAAGCgAGGUCAaCGCUGAc -3' miRNA: 3'- uUGAGCAUUUCG-UUCAGUcGCGACUa -5' |
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| 22913 | 5' | -50.2 | NC_005137.2 | + | 127001 | 1.04 | 0.0127 |
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Target: 5'- aAACUCGUAAAGCAAGUCAGCGCUGAUg -3' miRNA: 3'- -UUGAGCAUUUCGUUCAGUCGCGACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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