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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22915 | 3' | -47.3 | NC_005137.2 | + | 40615 | 0.66 | 0.999454 |
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Target: 5'- uGCUgaCCGAAUUuUACAAuucgcaauuGCGCUCCAAGu -3' miRNA: 3'- -CGA--GGUUUAGcAUGUU---------CGUGAGGUUU- -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 100692 | 0.66 | 0.999151 |
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Target: 5'- aGCUCCGAccUCGUcuuuCGGGCACggCCGu- -3' miRNA: 3'- -CGAGGUUu-AGCAu---GUUCGUGa-GGUuu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 50241 | 0.67 | 0.997705 |
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Target: 5'- aGCUCgGGGUCGUcggAUAAGC-CUUCGAu -3' miRNA: 3'- -CGAGgUUUAGCA---UGUUCGuGAGGUUu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 22565 | 0.68 | 0.995406 |
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Target: 5'- cGCUCCGcagCGU-CAGGCGCgCCAu- -3' miRNA: 3'- -CGAGGUuuaGCAuGUUCGUGaGGUuu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 95416 | 0.68 | 0.995406 |
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Target: 5'- -gUCCGcAUCGUGCAucuGCACUaCCGu- -3' miRNA: 3'- cgAGGUuUAGCAUGUu--CGUGA-GGUuu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 29689 | 0.68 | 0.994604 |
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Target: 5'- aGCUCCGGGUUGUugucGCGGcGCAC-CCAc- -3' miRNA: 3'- -CGAGGUUUAGCA----UGUU-CGUGaGGUuu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 105851 | 0.69 | 0.98714 |
|
Target: 5'- uGCUCCAGAcacggaggCGUGCGAGUuggCCGAAu -3' miRNA: 3'- -CGAGGUUUa-------GCAUGUUCGugaGGUUU- -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 3915 | 0.69 | 0.990204 |
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Target: 5'- cGCUCUAuguAAUCGUACaAAGCG-UCCAu- -3' miRNA: 3'- -CGAGGU---UUAGCAUG-UUCGUgAGGUuu -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 42515 | 0.69 | 0.990204 |
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Target: 5'- cGCUCCGGGUUGUcgGGGCAUUugcCCAGAc -3' miRNA: 3'- -CGAGGUUUAGCAugUUCGUGA---GGUUU- -5' |
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| 22915 | 3' | -47.3 | NC_005137.2 | + | 1110 | 1.1 | 0.013625 |
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Target: 5'- cGCUCCAAAUCGUACAAGCACUCCAAAu -3' miRNA: 3'- -CGAGGUUUAGCAUGUUCGUGAGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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