Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22915 | 5' | -51.7 | NC_005137.2 | + | 13984 | 0.66 | 0.968816 |
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Target: 5'- ---aCCAGuGCUCGCGCaCGUucUUUGGc -3' miRNA: 3'- aaaaGGUU-CGAGCGCGgGCA--AAACCu -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 11635 | 0.67 | 0.949923 |
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Target: 5'- ---aCCGAGCUCgGCGgCgCGUUUaGGAg -3' miRNA: 3'- aaaaGGUUCGAG-CGCgG-GCAAAaCCU- -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 7491 | 0.68 | 0.940595 |
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Target: 5'- ----gCAAGCUUGUGCCCGUUg---- -3' miRNA: 3'- aaaagGUUCGAGCGCGGGCAAaaccu -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 130590 | 0.68 | 0.935536 |
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Target: 5'- uUUUUCUgAGGgUUGCGCCCGUucuaaUUUGGu -3' miRNA: 3'- -AAAAGG-UUCgAGCGCGGGCA-----AAACCu -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 64247 | 0.69 | 0.899607 |
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Target: 5'- --gUCCGGGCaUCGCGCCaCGacaugUUGGu -3' miRNA: 3'- aaaAGGUUCG-AGCGCGG-GCaa---AACCu -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 98135 | 0.71 | 0.828762 |
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Target: 5'- ---cCCAcGCcgCGCGCCCGUUUUGc- -3' miRNA: 3'- aaaaGGUuCGa-GCGCGGGCAAAACcu -5' |
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| 22915 | 5' | -51.7 | NC_005137.2 | + | 1145 | 1.07 | 0.006828 |
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Target: 5'- gUUUUCCAAGCUCGCGCCCGUUUUGGAg -3' miRNA: 3'- -AAAAGGUUCGAGCGCGGGCAAAACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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