Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22916 | 3' | -51.2 | NC_005137.2 | + | 4284 | 1.1 | 0.006212 |
|
Target: 5'- aCUAAAUAGCCGGUGCGGUUAAGGCCGu -3' miRNA: 3'- -GAUUUAUCGGCCACGCCAAUUCCGGC- -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 108874 | 0.7 | 0.898127 |
|
Target: 5'- aUGGAUAGCgGGgcaacgcuugacGCGGUUcuuacAAGGCCGc -3' miRNA: 3'- gAUUUAUCGgCCa-----------CGCCAA-----UUCCGGC- -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 12372 | 0.69 | 0.929866 |
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Target: 5'- ---cAUAGCaCGGUGCGGUUGuccaguuuGGCa- -3' miRNA: 3'- gauuUAUCG-GCCACGCCAAUu-------CCGgc -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 117581 | 0.68 | 0.94497 |
|
Target: 5'- ------cGCCGGUGCGGcUGAcGGgCGa -3' miRNA: 3'- gauuuauCGGCCACGCCaAUU-CCgGC- -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 70152 | 0.67 | 0.965065 |
|
Target: 5'- aCUAGGUGG-CGGUGuCGGUgAAGGUg- -3' miRNA: 3'- -GAUUUAUCgGCCAC-GCCAaUUCCGgc -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 34817 | 0.73 | 0.76352 |
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Target: 5'- ------uGCUGGUGCGGUggcGGGUCGg -3' miRNA: 3'- gauuuauCGGCCACGCCAau-UCCGGC- -5' |
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| 22916 | 3' | -51.2 | NC_005137.2 | + | 64887 | 0.77 | 0.542684 |
|
Target: 5'- aUGGGUucGCCGGUGaacgGGUUGAGGCCu -3' miRNA: 3'- gAUUUAu-CGGCCACg---CCAAUUCCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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