miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22916 3' -51.2 NC_005137.2 + 4284 1.1 0.006212
Target:  5'- aCUAAAUAGCCGGUGCGGUUAAGGCCGu -3'
miRNA:   3'- -GAUUUAUCGGCCACGCCAAUUCCGGC- -5'
22916 3' -51.2 NC_005137.2 + 108874 0.7 0.898127
Target:  5'- aUGGAUAGCgGGgcaacgcuugacGCGGUUcuuacAAGGCCGc -3'
miRNA:   3'- gAUUUAUCGgCCa-----------CGCCAA-----UUCCGGC- -5'
22916 3' -51.2 NC_005137.2 + 12372 0.69 0.929866
Target:  5'- ---cAUAGCaCGGUGCGGUUGuccaguuuGGCa- -3'
miRNA:   3'- gauuUAUCG-GCCACGCCAAUu-------CCGgc -5'
22916 3' -51.2 NC_005137.2 + 117581 0.68 0.94497
Target:  5'- ------cGCCGGUGCGGcUGAcGGgCGa -3'
miRNA:   3'- gauuuauCGGCCACGCCaAUU-CCgGC- -5'
22916 3' -51.2 NC_005137.2 + 70152 0.67 0.965065
Target:  5'- aCUAGGUGG-CGGUGuCGGUgAAGGUg- -3'
miRNA:   3'- -GAUUUAUCgGCCAC-GCCAaUUCCGgc -5'
22916 3' -51.2 NC_005137.2 + 34817 0.73 0.76352
Target:  5'- ------uGCUGGUGCGGUggcGGGUCGg -3'
miRNA:   3'- gauuuauCGGCCACGCCAau-UCCGGC- -5'
22916 3' -51.2 NC_005137.2 + 64887 0.77 0.542684
Target:  5'- aUGGGUucGCCGGUGaacgGGUUGAGGCCu -3'
miRNA:   3'- gAUUUAu-CGGCCACg---CCAAUUCCGGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.