Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22917 | 5' | -53.8 | NC_005137.2 | + | 27942 | 0.68 | 0.872053 |
Target: 5'- cGCACc----GCUUGCGGaaaGCGUGACa -3' miRNA: 3'- -CGUGcauuaCGGACGCCg--CGUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100407 | 0.68 | 0.886695 |
Target: 5'- cGCGCGcccGUGCgUUGCGGCGCccgGAUc -3' miRNA: 3'- -CGUGCau-UACG-GACGCCGCGua-CUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 63450 | 0.67 | 0.900379 |
Target: 5'- cGCGCGUuGUGCCcGCGaGCGac-GGCg -3' miRNA: 3'- -CGUGCAuUACGGaCGC-CGCguaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 104689 | 0.67 | 0.919044 |
Target: 5'- -aACGUGG-GCC-GCGGCGUGUGGgUg -3' miRNA: 3'- cgUGCAUUaCGGaCGCCGCGUACUgA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 48947 | 0.66 | 0.92476 |
Target: 5'- cGCaAUGUAGUGCCgcacacgcugUGCGGCGCc--GCg -3' miRNA: 3'- -CG-UGCAUUACGG----------ACGCCGCGuacUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 70179 | 0.66 | 0.929687 |
Target: 5'- cGUACGcGGUGCCgugggauUGcCGGCGgGUGAUa -3' miRNA: 3'- -CGUGCaUUACGG-------AC-GCCGCgUACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 120104 | 0.66 | 0.930222 |
Target: 5'- -aGCGU--UGCUUGC-GCGUGUGGCUg -3' miRNA: 3'- cgUGCAuuACGGACGcCGCGUACUGA- -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 100141 | 0.66 | 0.935428 |
Target: 5'- gGUugGUGcGUGCgCUGCuGGCGCAcGAa- -3' miRNA: 3'- -CGugCAU-UACG-GACG-CCGCGUaCUga -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 95333 | 0.66 | 0.935428 |
Target: 5'- cGUGCGUAGUGCa--CGGCGUcaaugugcgGUGGCa -3' miRNA: 3'- -CGUGCAUUACGgacGCCGCG---------UACUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 78065 | 0.66 | 0.935428 |
Target: 5'- uGCAgGUGgguGUGUUUGCGGuUGCAcGACg -3' miRNA: 3'- -CGUgCAU---UACGGACGCC-GCGUaCUGa -5' |
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22917 | 5' | -53.8 | NC_005137.2 | + | 2639 | 0.66 | 0.940382 |
Target: 5'- aGCACc--AUGCCgcacGCGGCGCAUa--- -3' miRNA: 3'- -CGUGcauUACGGa---CGCCGCGUAcuga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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