Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22918 | 3' | -46.3 | NC_005137.2 | + | 37616 | 0.66 | 0.999816 |
Target: 5'- --cUUUGACCGgaucgaGCGCAaauacgUGGUgCGCGg -3' miRNA: 3'- gaaAAACUGGCa-----CGCGUa-----ACUA-GUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 118734 | 0.66 | 0.999816 |
Target: 5'- -gUUUUGGCaaUGCGCAguccgUGAUCAgGc -3' miRNA: 3'- gaAAAACUGgcACGCGUa----ACUAGUgC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 27627 | 0.66 | 0.999858 |
Target: 5'- ---aUUGACUGgcgGCGCAc--GUCGCGa -3' miRNA: 3'- gaaaAACUGGCa--CGCGUaacUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 66313 | 0.66 | 0.999918 |
Target: 5'- ------aGCCGaUGCGCGUaccaauugUGGUCGCGu -3' miRNA: 3'- gaaaaacUGGC-ACGCGUA--------ACUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 107986 | 0.66 | 0.999939 |
Target: 5'- ---gUUGACgCGcGCGCAgcUUGAUCAa- -3' miRNA: 3'- gaaaAACUG-GCaCGCGU--AACUAGUgc -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 110458 | 0.66 | 0.999816 |
Target: 5'- -----cGGCCG-GCGCGUacacggGGUCACu -3' miRNA: 3'- gaaaaaCUGGCaCGCGUAa-----CUAGUGc -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 51822 | 0.66 | 0.999918 |
Target: 5'- ------uGCCGuUGCGCuuaaacUUGAUCACGu -3' miRNA: 3'- gaaaaacUGGC-ACGCGu-----AACUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 55260 | 0.66 | 0.999918 |
Target: 5'- --cUUUGACCGUGUGC--UGA-CAUu -3' miRNA: 3'- gaaAAACUGGCACGCGuaACUaGUGc -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 2248 | 0.66 | 0.999939 |
Target: 5'- --aUUUGGCgCGauugcGCGCGUUGAUUgACGg -3' miRNA: 3'- gaaAAACUG-GCa----CGCGUAACUAG-UGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 77054 | 0.67 | 0.999762 |
Target: 5'- ----cUGAUCGUGauaGCGUUGccaAUCGCGc -3' miRNA: 3'- gaaaaACUGGCACg--CGUAAC---UAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 129064 | 0.67 | 0.999762 |
Target: 5'- ------aAUUGUGCGCGUUGAUCGu- -3' miRNA: 3'- gaaaaacUGGCACGCGUAACUAGUgc -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 112547 | 0.68 | 0.999388 |
Target: 5'- ------cGCCGUGCGC---GAUCGCGc -3' miRNA: 3'- gaaaaacUGGCACGCGuaaCUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 118556 | 0.68 | 0.999388 |
Target: 5'- -----cGACCGUggacaugaaaGCGCc-UGAUCACGg -3' miRNA: 3'- gaaaaaCUGGCA----------CGCGuaACUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 12852 | 0.68 | 0.998584 |
Target: 5'- -----aGGCCGUagcccgGCGCAaucugGAUCACGg -3' miRNA: 3'- gaaaaaCUGGCA------CGCGUaa---CUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 75968 | 0.7 | 0.99582 |
Target: 5'- ----gUGACgCGgcGCGCGUUGGUgCACGg -3' miRNA: 3'- gaaaaACUG-GCa-CGCGUAACUA-GUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 60767 | 0.7 | 0.994246 |
Target: 5'- --aUUUGGcCCGUGCGCGUU--UCGCc -3' miRNA: 3'- gaaAAACU-GGCACGCGUAAcuAGUGc -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 77222 | 0.72 | 0.984687 |
Target: 5'- --cUUUGcCCGUGCGCgAUUGGcaacgcuaUCACGa -3' miRNA: 3'- gaaAAACuGGCACGCG-UAACU--------AGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 93728 | 0.73 | 0.975413 |
Target: 5'- -----gGugCGUGCGCGUcaauuaaagcguUGGUCGCGc -3' miRNA: 3'- gaaaaaCugGCACGCGUA------------ACUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 42422 | 0.75 | 0.930203 |
Target: 5'- ----aUGACCGUGaGCAU-GGUCACGu -3' miRNA: 3'- gaaaaACUGGCACgCGUAaCUAGUGC- -5' |
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22918 | 3' | -46.3 | NC_005137.2 | + | 10835 | 1.09 | 0.021456 |
Target: 5'- uCUUUUUGACCGUGCGCAUUGAUCACGc -3' miRNA: 3'- -GAAAAACUGGCACGCGUAACUAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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