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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22918 | 5' | -53.9 | NC_005137.2 | + | 36634 | 0.66 | 0.940579 |
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Target: 5'- cGUUGUGACGGUugacgGCAC-CGuUGACUUu -3' miRNA: 3'- -CAGCAUUGCCGa----CGUGuGCcACUGAA- -5' |
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| 22918 | 5' | -53.9 | NC_005137.2 | + | 63918 | 0.68 | 0.864296 |
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Target: 5'- cUUGgcgGACGGUUcGCACGCGGUGcGCg- -3' miRNA: 3'- cAGCa--UUGCCGA-CGUGUGCCAC-UGaa -5' |
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| 22918 | 5' | -53.9 | NC_005137.2 | + | 98523 | 0.69 | 0.822555 |
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Target: 5'- cGUCGcGACGGCgccaaaccgGCGCacaagcgugACGGUGACg- -3' miRNA: 3'- -CAGCaUUGCCGa--------CGUG---------UGCCACUGaa -5' |
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| 22918 | 5' | -53.9 | NC_005137.2 | + | 130402 | 0.7 | 0.763508 |
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Target: 5'- --gGUGGCGGCgGCACguuuggcggcggagGCGGUGGCa- -3' miRNA: 3'- cagCAUUGCCGaCGUG--------------UGCCACUGaa -5' |
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| 22918 | 5' | -53.9 | NC_005137.2 | + | 10869 | 1.06 | 0.005551 |
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Target: 5'- uGUCGUAACGGCUGCACACGGUGACUUg -3' miRNA: 3'- -CAGCAUUGCCGACGUGUGCCACUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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