miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22918 5' -53.9 NC_005137.2 + 10869 1.06 0.005551
Target:  5'- uGUCGUAACGGCUGCACACGGUGACUUg -3'
miRNA:   3'- -CAGCAUUGCCGACGUGUGCCACUGAA- -5'
22918 5' -53.9 NC_005137.2 + 130402 0.7 0.763508
Target:  5'- --gGUGGCGGCgGCACguuuggcggcggagGCGGUGGCa- -3'
miRNA:   3'- cagCAUUGCCGaCGUG--------------UGCCACUGaa -5'
22918 5' -53.9 NC_005137.2 + 98523 0.69 0.822555
Target:  5'- cGUCGcGACGGCgccaaaccgGCGCacaagcgugACGGUGACg- -3'
miRNA:   3'- -CAGCaUUGCCGa--------CGUG---------UGCCACUGaa -5'
22918 5' -53.9 NC_005137.2 + 63918 0.68 0.864296
Target:  5'- cUUGgcgGACGGUUcGCACGCGGUGcGCg- -3'
miRNA:   3'- cAGCa--UUGCCGA-CGUGUGCCAC-UGaa -5'
22918 5' -53.9 NC_005137.2 + 36634 0.66 0.940579
Target:  5'- cGUUGUGACGGUugacgGCAC-CGuUGACUUu -3'
miRNA:   3'- -CAGCAUUGCCGa----CGUGuGCcACUGAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.