Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22921 | 3' | -53 | NC_005137.2 | + | 48773 | 0.66 | 0.954401 |
Target: 5'- -aUCGuUGGAcGCGGCGCCGcacagcgugugcGGCAc- -3' miRNA: 3'- gaAGUuGCCUuUGCCGCGGU------------CCGUuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 310 | 0.66 | 0.954401 |
Target: 5'- --cCAGCGGAGAgCGuguuGCGCCAGGa--- -3' miRNA: 3'- gaaGUUGCCUUU-GC----CGCGGUCCguuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 99523 | 0.66 | 0.95018 |
Target: 5'- --aCAACGGGuuuGCgGGCGCCGuGCAu- -3' miRNA: 3'- gaaGUUGCCUu--UG-CCGCGGUcCGUuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 32199 | 0.66 | 0.945706 |
Target: 5'- -gUCGGCGcacGGCGGCGUuuGGCAGAc -3' miRNA: 3'- gaAGUUGCcu-UUGCCGCGguCCGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 55578 | 0.66 | 0.940976 |
Target: 5'- ---uGGCGGAcgcgGGCGGCGCCGuGCGc- -3' miRNA: 3'- gaagUUGCCU----UUGCCGCGGUcCGUuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 19706 | 0.66 | 0.940976 |
Target: 5'- uUUCAgACGGGu-UGGCGgCGGGCAc- -3' miRNA: 3'- gAAGU-UGCCUuuGCCGCgGUCCGUuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 24398 | 0.67 | 0.913423 |
Target: 5'- -----uUGGAcGCGGCGUCAGGCc-- -3' miRNA: 3'- gaaguuGCCUuUGCCGCGGUCCGuuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 105497 | 0.68 | 0.900583 |
Target: 5'- --aCGACGGuuaucuagaauuAAGCGGCGCCAcacGGCu-- -3' miRNA: 3'- gaaGUUGCC------------UUUGCCGCGGU---CCGuuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 89105 | 0.68 | 0.886726 |
Target: 5'- -aUC-ACGGAGAuUGGCGC-GGGCGAAa -3' miRNA: 3'- gaAGuUGCCUUU-GCCGCGgUCCGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 45676 | 0.68 | 0.886726 |
Target: 5'- --aCGugGGcgGCGGCGCCAacGCGGAu -3' miRNA: 3'- gaaGUugCCuuUGCCGCGGUc-CGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 46640 | 0.68 | 0.879427 |
Target: 5'- --aUggUGGAcAGCGGCGCUaaGGGCAGc -3' miRNA: 3'- gaaGuuGCCU-UUGCCGCGG--UCCGUUu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 88172 | 0.68 | 0.871887 |
Target: 5'- -cUCGGCGGugGCcGCGCC-GGCAAu -3' miRNA: 3'- gaAGUUGCCuuUGcCGCGGuCCGUUu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 106207 | 0.68 | 0.871887 |
Target: 5'- --aCGugGGAcGCGGuCGCCaagaacGGGCAAAa -3' miRNA: 3'- gaaGUugCCUuUGCC-GCGG------UCCGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 110719 | 0.69 | 0.861735 |
Target: 5'- --gCAACGGuguGCGcgcagagcgcuuguGCGCCGGGCGAu -3' miRNA: 3'- gaaGUUGCCuu-UGC--------------CGCGGUCCGUUu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 3407 | 0.69 | 0.847884 |
Target: 5'- --gCGACGGuaccacguuuACGGCcaucGCCGGGCAAAa -3' miRNA: 3'- gaaGUUGCCuu--------UGCCG----CGGUCCGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 108612 | 0.7 | 0.819267 |
Target: 5'- gCUUCAagcacaaccuggugGCGGuGACGGUGCCAcGCGAc -3' miRNA: 3'- -GAAGU--------------UGCCuUUGCCGCGGUcCGUUu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 114271 | 0.7 | 0.81656 |
Target: 5'- uUUCAucCGGAAgcggugcagacgcacGCGGCGCUcGGCGAAc -3' miRNA: 3'- gAAGUu-GCCUU---------------UGCCGCGGuCCGUUU- -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 78811 | 0.7 | 0.784794 |
Target: 5'- ---gAGCGGGAGCGGCGUCGucGGUAu- -3' miRNA: 3'- gaagUUGCCUUUGCCGCGGU--CCGUuu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 18409 | 0.7 | 0.775103 |
Target: 5'- -gUUAACGGAcGCGGCGCC--GCAGu -3' miRNA: 3'- gaAGUUGCCUuUGCCGCGGucCGUUu -5' |
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22921 | 3' | -53 | NC_005137.2 | + | 125045 | 0.71 | 0.765272 |
Target: 5'- --gCAACGGAGccgggcggcGCGGCGCCGGaucugaacggcGCAAAg -3' miRNA: 3'- gaaGUUGCCUU---------UGCCGCGGUC-----------CGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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