Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22922 | 5' | -62.5 | NC_005137.2 | + | 97979 | 0.66 | 0.577994 |
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Target: 5'- uAGGCAAaaCGGGC-GCgCGGcgUGGGUg -3' miRNA: 3'- -UCCGUUgaGCCCGcCG-GCCa-ACCCGa -5' |
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| 22922 | 5' | -62.5 | NC_005137.2 | + | 125042 | 0.66 | 0.575038 |
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Target: 5'- cAGGCAACggagcCGGGCGGCgCGGcgccggaucugaacGGCg -3' miRNA: 3'- -UCCGUUGa----GCCCGCCG-GCCaac-----------CCGa -5' |
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| 22922 | 5' | -62.5 | NC_005137.2 | + | 61352 | 0.67 | 0.500764 |
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Target: 5'- uGGCAcgcGCUCugcgucaaauGGGCGGCCGGcgagUUGauGGCg -3' miRNA: 3'- uCCGU---UGAG----------CCCGCCGGCC----AAC--CCGa -5' |
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| 22922 | 5' | -62.5 | NC_005137.2 | + | 107132 | 0.69 | 0.394635 |
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Target: 5'- -aGCGACgaagGcGGCGGCgGGUUGGGUg -3' miRNA: 3'- ucCGUUGag--C-CCGCCGgCCAACCCGa -5' |
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| 22922 | 5' | -62.5 | NC_005137.2 | + | 122531 | 0.74 | 0.204546 |
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Target: 5'- aAGGCGGCgggcgUGGGCgugcuauuGGCCGGagGGGCUu -3' miRNA: 3'- -UCCGUUGa----GCCCG--------CCGGCCaaCCCGA- -5' |
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| 22922 | 5' | -62.5 | NC_005137.2 | + | 22612 | 1.07 | 0.000775 |
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Target: 5'- cAGGCAACUCGGGCGGCCGGUUGGGCUg -3' miRNA: 3'- -UCCGUUGAGCCCGCCGGCCAACCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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