miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22922 5' -62.5 NC_005137.2 + 97979 0.66 0.577994
Target:  5'- uAGGCAAaaCGGGC-GCgCGGcgUGGGUg -3'
miRNA:   3'- -UCCGUUgaGCCCGcCG-GCCa-ACCCGa -5'
22922 5' -62.5 NC_005137.2 + 125042 0.66 0.575038
Target:  5'- cAGGCAACggagcCGGGCGGCgCGGcgccggaucugaacGGCg -3'
miRNA:   3'- -UCCGUUGa----GCCCGCCG-GCCaac-----------CCGa -5'
22922 5' -62.5 NC_005137.2 + 61352 0.67 0.500764
Target:  5'- uGGCAcgcGCUCugcgucaaauGGGCGGCCGGcgagUUGauGGCg -3'
miRNA:   3'- uCCGU---UGAG----------CCCGCCGGCC----AAC--CCGa -5'
22922 5' -62.5 NC_005137.2 + 107132 0.69 0.394635
Target:  5'- -aGCGACgaagGcGGCGGCgGGUUGGGUg -3'
miRNA:   3'- ucCGUUGag--C-CCGCCGgCCAACCCGa -5'
22922 5' -62.5 NC_005137.2 + 122531 0.74 0.204546
Target:  5'- aAGGCGGCgggcgUGGGCgugcuauuGGCCGGagGGGCUu -3'
miRNA:   3'- -UCCGUUGa----GCCCG--------CCGGCCaaCCCGA- -5'
22922 5' -62.5 NC_005137.2 + 22612 1.07 0.000775
Target:  5'- cAGGCAACUCGGGCGGCCGGUUGGGCUg -3'
miRNA:   3'- -UCCGUUGAGCCCGCCGGCCAACCCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.