Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22923 | 3' | -53 | NC_005137.2 | + | 28048 | 0.69 | 0.855695 |
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Target: 5'- -cGUGGCGcGAC-GCGCGCUGGgaugcgcCAGACg -3' miRNA: 3'- gaCAUUGC-UUGcCGCGCGACU-------GUCUG- -5' |
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| 22923 | 3' | -53 | NC_005137.2 | + | 12280 | 0.7 | 0.84839 |
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Target: 5'- -gGUGGuuaaGGACGGCGCGUUGAUuaaaccauuuuuGGGCa -3' miRNA: 3'- gaCAUUg---CUUGCCGCGCGACUG------------UCUG- -5' |
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| 22923 | 3' | -53 | NC_005137.2 | + | 87325 | 0.7 | 0.813955 |
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Target: 5'- -cGUcGCGaAACGGCGCaGCUGGCcaaccgcauggcGGACa -3' miRNA: 3'- gaCAuUGC-UUGCCGCG-CGACUG------------UCUG- -5' |
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| 22923 | 3' | -53 | NC_005137.2 | + | 11417 | 0.73 | 0.685847 |
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Target: 5'- -cGUAGCGGACGucaGCGCGCUGGaggGGAg -3' miRNA: 3'- gaCAUUGCUUGC---CGCGCGACUg--UCUg -5' |
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| 22923 | 3' | -53 | NC_005137.2 | + | 55897 | 0.76 | 0.518761 |
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Target: 5'- aUGUGG-GAcACGGUGCGCUGGCAGGu -3' miRNA: 3'- gACAUUgCU-UGCCGCGCGACUGUCUg -5' |
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| 22923 | 3' | -53 | NC_005137.2 | + | 24281 | 1.11 | 0.003305 |
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Target: 5'- aCUGUAACGAACGGCGCGCUGACAGACg -3' miRNA: 3'- -GACAUUGCUUGCCGCGCGACUGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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