Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22925 | 3' | -57.3 | NC_005137.2 | + | 27435 | 1.1 | 0.001406 |
Target: 5'- cAGCUGGCCCACGCGCUUGUCUCGUACg -3' miRNA: 3'- -UCGACCGGGUGCGCGAACAGAGCAUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 32229 | 0.67 | 0.739078 |
Target: 5'- --aUGGCCUugGCaagacGCUUGUCggGUGCu -3' miRNA: 3'- ucgACCGGGugCG-----CGAACAGagCAUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 99193 | 0.67 | 0.758592 |
Target: 5'- cGUUGccGCCCACGUGCcg--CUUGUACa -3' miRNA: 3'- uCGAC--CGGGUGCGCGaacaGAGCAUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 103987 | 0.67 | 0.768181 |
Target: 5'- cAGCUcaaGGUUCAUGCGCagcaUGUCguucUCGUACa -3' miRNA: 3'- -UCGA---CCGGGUGCGCGa---ACAG----AGCAUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 72800 | 0.66 | 0.8228 |
Target: 5'- cGUUGGCggCGCGCGCUgccGUCUUGa-- -3' miRNA: 3'- uCGACCGg-GUGCGCGAa--CAGAGCaug -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 55040 | 0.66 | 0.8228 |
Target: 5'- cAGUUGG-CCACGUGUUUGUCgg--ACa -3' miRNA: 3'- -UCGACCgGGUGCGCGAACAGagcaUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 113265 | 0.66 | 0.80521 |
Target: 5'- cGUUGGCgCACGCGCUUGcCa----- -3' miRNA: 3'- uCGACCGgGUGCGCGAACaGagcaug -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 130776 | 0.66 | 0.78698 |
Target: 5'- uGCUGGCUUugucgGCGCGCgUGUC-CGcGCu -3' miRNA: 3'- uCGACCGGG-----UGCGCGaACAGaGCaUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 38260 | 0.67 | 0.758592 |
Target: 5'- aAGCUGGgaaCCGCGUugcccguggGCUUGUC-CGUGg -3' miRNA: 3'- -UCGACCg--GGUGCG---------CGAACAGaGCAUg -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 7780 | 0.67 | 0.739078 |
Target: 5'- cGCUGGCCgGCGaaacucugaaaUGCaUGUC-CGUACc -3' miRNA: 3'- uCGACCGGgUGC-----------GCGaACAGaGCAUG- -5' |
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22925 | 3' | -57.3 | NC_005137.2 | + | 108054 | 0.68 | 0.678558 |
Target: 5'- aAGCUGGgCUACGUGCaUUGUCgu-UACg -3' miRNA: 3'- -UCGACCgGGUGCGCG-AACAGagcAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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