Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22926 | 5' | -52.6 | NC_005137.2 | + | 27978 | 1.11 | 0.003814 |
Target: 5'- uGUGAAACAUGCCCGCUUCCGCGAUGGa -3' miRNA: 3'- -CACUUUGUACGGGCGAAGGCGCUACC- -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 2438 | 0.71 | 0.813955 |
Target: 5'- gGUGGccGACGUGCCCGCgcCCGaCGAc-- -3' miRNA: 3'- -CACU--UUGUACGGGCGaaGGC-GCUacc -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 88946 | 0.69 | 0.872053 |
Target: 5'- ----cGCGUuucGCCCGCgccaaucUCCGUGAUGGg -3' miRNA: 3'- cacuuUGUA---CGGGCGa------AGGCGCUACC- -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 89106 | 0.68 | 0.919044 |
Target: 5'- --cAAACAgucUGUCCGCUUCCGUGGg-- -3' miRNA: 3'- cacUUUGU---ACGGGCGAAGGCGCUacc -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 77306 | 0.67 | 0.957315 |
Target: 5'- uGUGGccaaaacGGCcgGCUCGuCUUCCGCGGUc- -3' miRNA: 3'- -CACU-------UUGuaCGGGC-GAAGGCGCUAcc -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 5581 | 0.66 | 0.964913 |
Target: 5'- -gGAGACGUGUauggCCGUUUuuGCGGcauUGGc -3' miRNA: 3'- caCUUUGUACG----GGCGAAggCGCU---ACC- -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 38255 | 0.66 | 0.974042 |
Target: 5'- -gGGAACcgcguUGCCCGUgggcuugUCCGUGgcGGu -3' miRNA: 3'- caCUUUGu----ACGGGCGa------AGGCGCuaCC- -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 96036 | 0.66 | 0.961423 |
Target: 5'- -cGAcGCAccaUGCCCGUgUCgGCGGUGu -3' miRNA: 3'- caCUuUGU---ACGGGCGaAGgCGCUACc -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 21311 | 0.67 | 0.945083 |
Target: 5'- aUGccACAUGCCCGCgugcggUuuGCGGUa- -3' miRNA: 3'- cACuuUGUACGGGCGa-----AggCGCUAcc -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 50848 | 0.7 | 0.840075 |
Target: 5'- --aAGACGUGCCCGCcaaCGCGAuuuguuUGGg -3' miRNA: 3'- cacUUUGUACGGGCGaagGCGCU------ACC- -5' |
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22926 | 5' | -52.6 | NC_005137.2 | + | 88185 | 0.71 | 0.822849 |
Target: 5'- cGUGGug---GCCCGCcUCgGCGGUGGc -3' miRNA: 3'- -CACUuuguaCGGGCGaAGgCGCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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