Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22928 | 3' | -57.5 | NC_005137.2 | + | 59792 | 0.66 | 0.820116 |
Target: 5'- aCAgcgGCGuGCCGuUGCAaaaaGUGGCgcGCUUGa -3' miRNA: 3'- -GUa--UGC-CGGC-ACGUg---CGCCGa-CGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 22448 | 0.66 | 0.820116 |
Target: 5'- -uUugGGCaaCGUGCGCuccguCGGCUGCa-- -3' miRNA: 3'- guAugCCG--GCACGUGc----GCCGACGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 125295 | 0.66 | 0.814893 |
Target: 5'- --aGCGGCgGUGCAUugucaagacguucgaGCGGCgcggcccgccGCUUGa -3' miRNA: 3'- guaUGCCGgCACGUG---------------CGCCGa---------CGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 19989 | 0.66 | 0.811377 |
Target: 5'- --gGCGGCgGagGCuuuguguCGCGGCguUGCUUGg -3' miRNA: 3'- guaUGCCGgCa-CGu------GCGCCG--ACGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 28077 | 0.66 | 0.811377 |
Target: 5'- --gACGGCUGUacacGCAgCGCGGcCUGCc-- -3' miRNA: 3'- guaUGCCGGCA----CGU-GCGCC-GACGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 70581 | 0.66 | 0.811377 |
Target: 5'- uCAUGCGuuCGUGCuGCGCGgGCgGCUUu -3' miRNA: 3'- -GUAUGCcgGCACG-UGCGC-CGaCGAAc -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 106862 | 0.66 | 0.793413 |
Target: 5'- --aACGGCgGcGUuuGCGGCUGCggUGg -3' miRNA: 3'- guaUGCCGgCaCGugCGCCGACGa-AC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 33766 | 0.66 | 0.793413 |
Target: 5'- --cAUGGCCauuGUGCGCgGCGGCguugUGCUg- -3' miRNA: 3'- guaUGCCGG---CACGUG-CGCCG----ACGAac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 62292 | 0.66 | 0.793413 |
Target: 5'- --aACGGUCGcGCugGUGGgUGCa-- -3' miRNA: 3'- guaUGCCGGCaCGugCGCCgACGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 31634 | 0.66 | 0.793413 |
Target: 5'- --aACGGUCaGUGCACGCGcGCggacGCg-- -3' miRNA: 3'- guaUGCCGG-CACGUGCGC-CGa---CGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 100038 | 0.66 | 0.774858 |
Target: 5'- --cGCGGuCCGacgcgGCGCGCGGC-GCg-- -3' miRNA: 3'- guaUGCC-GGCa----CGUGCGCCGaCGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 111511 | 0.67 | 0.755784 |
Target: 5'- gCAUAaauucgaGGCgCGUGUACaGCGaGCUGCUg- -3' miRNA: 3'- -GUAUg------CCG-GCACGUG-CGC-CGACGAac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 95371 | 0.67 | 0.755784 |
Target: 5'- aGUACGGgCGUGU----GGCUGCUUGc -3' miRNA: 3'- gUAUGCCgGCACGugcgCCGACGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 32195 | 0.67 | 0.746077 |
Target: 5'- --cGCGGUCGgcGCACgGCGGC-GUUUGg -3' miRNA: 3'- guaUGCCGGCa-CGUG-CGCCGaCGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 112865 | 0.67 | 0.744123 |
Target: 5'- -cUGCGGCuCGguccaagugauaGCACGCGGgCUGCg-- -3' miRNA: 3'- guAUGCCG-GCa-----------CGUGCGCC-GACGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 75246 | 0.67 | 0.726366 |
Target: 5'- --gAUGGCCGUGaC-CGCGGgC-GCUUGa -3' miRNA: 3'- guaUGCCGGCAC-GuGCGCC-GaCGAAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 101156 | 0.68 | 0.71538 |
Target: 5'- uGUACGGCCGcgGUuggacagGCGUGGCagGCUa- -3' miRNA: 3'- gUAUGCCGGCa-CG-------UGCGCCGa-CGAac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 112443 | 0.69 | 0.644965 |
Target: 5'- aGU-CGGCCGuUGgACGCGGCgGCa-- -3' miRNA: 3'- gUAuGCCGGC-ACgUGCGCCGaCGaac -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 75443 | 0.69 | 0.644965 |
Target: 5'- -uUGCGGCCaacuUGCACGUGGCcgugGCaUUGu -3' miRNA: 3'- guAUGCCGGc---ACGUGCGCCGa---CG-AAC- -5' |
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22928 | 3' | -57.5 | NC_005137.2 | + | 117999 | 0.69 | 0.624336 |
Target: 5'- --cGCGuCCGUGCugGUGGUUGCc-- -3' miRNA: 3'- guaUGCcGGCACGugCGCCGACGaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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