miRNA: 3'- gCGGUUGUUG-GCAACCACaUGGUggc -5'
miRNA: 3'- -GCGGUu----GUUGGCAACCACa----UGGUGGC- -5'
miRNA: 3'- -GCGGUUGUUGGCAACCACaUGGUGGC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22928 | 5' | -52.8 | NC_005137.2 | + | 57425 | 0.74 | 0.64225 |
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Target: 5'- gGCCAGCAACgCGUUGGUG-GCCGa-- -3' miRNA: 3'- gCGGUUGUUG-GCAACCACaUGGUggc -5' |
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| 22928 | 5' | -52.8 | NC_005137.2 | + | 50050 | 0.75 | 0.589908 |
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Target: 5'- aCGCCAucuugUAACCGUUGGUGaauugGCCAUUGa -3' miRNA: 3'- -GCGGUu----GUUGGCAACCACa----UGGUGGC- -5' |
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| 22928 | 5' | -52.8 | NC_005137.2 | + | 32588 | 0.97 | 0.033045 |
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Target: 5'- cCGCCAACAACCGUUGGUGcAUCACCGg -3' miRNA: 3'- -GCGGUUGUUGGCAACCACaUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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