Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22929 | 3' | -61.2 | NC_005137.2 | + | 48479 | 0.66 | 0.59481 |
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Target: 5'- -cGUGCcGCCAaggCCGuGCGC-GACCAGc -3' miRNA: 3'- uaCACGaCGGUg--GGC-CGCGaCUGGUU- -5' |
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| 22929 | 3' | -61.2 | NC_005137.2 | + | 88185 | 0.66 | 0.574433 |
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Target: 5'- -cGUGgUGgcCCGCCuCGGCGgUGGCCGc -3' miRNA: 3'- uaCACgAC--GGUGG-GCCGCgACUGGUu -5' |
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| 22929 | 3' | -61.2 | NC_005137.2 | + | 67688 | 0.66 | 0.534222 |
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Target: 5'- uUGUGCcGCCuCgCGGCGUUGACa-- -3' miRNA: 3'- uACACGaCGGuGgGCCGCGACUGguu -5' |
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| 22929 | 3' | -61.2 | NC_005137.2 | + | 3588 | 0.67 | 0.524318 |
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Target: 5'- uUGUGCacguaaGCCuuuuGCCCGGCGaUGGCCGu -3' miRNA: 3'- uACACGa-----CGG----UGGGCCGCgACUGGUu -5' |
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| 22929 | 3' | -61.2 | NC_005137.2 | + | 50998 | 0.69 | 0.395041 |
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Target: 5'- -cGUuuUuUCACCUGGCGCUGACCAAc -3' miRNA: 3'- uaCAcgAcGGUGGGCCGCGACUGGUU- -5' |
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| 22929 | 3' | -61.2 | NC_005137.2 | + | 32966 | 1.04 | 0.001312 |
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Target: 5'- aAUGUGCUGCCACCCGGCGCUGACCAAg -3' miRNA: 3'- -UACACGACGGUGGGCCGCGACUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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