Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22930 | 3' | -52 | NC_005137.2 | + | 46790 | 0.66 | 0.979286 |
Target: 5'- --cUUAGCGCCGCUGuC-CACCaugGCg -3' miRNA: 3'- ucaAAUUGCGGCGGCuGaGUGGca-UG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 128691 | 0.66 | 0.976873 |
Target: 5'- ----aAACGCCGCCaGCaauacaaugauaUCGCCGUGu -3' miRNA: 3'- ucaaaUUGCGGCGGcUG------------AGUGGCAUg -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 99805 | 0.66 | 0.976873 |
Target: 5'- gAGgu---CGCCGCgCGugUaCGCCGUAa -3' miRNA: 3'- -UCaaauuGCGGCG-GCugA-GUGGCAUg -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 62215 | 0.66 | 0.974259 |
Target: 5'- gAGUUU-GCGCUGCCGcgauCUguCCGcUGCa -3' miRNA: 3'- -UCAAAuUGCGGCGGCu---GAguGGC-AUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 71203 | 0.66 | 0.971437 |
Target: 5'- gAGUUUAugGacaagaCCGCCGAgCUCAauuugcgcUCGUGCc -3' miRNA: 3'- -UCAAAUugC------GGCGGCU-GAGU--------GGCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 55579 | 0.66 | 0.971437 |
Target: 5'- ---aUGGCGgaCGCgGGCggCGCCGUGCg -3' miRNA: 3'- ucaaAUUGCg-GCGgCUGa-GUGGCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 44075 | 0.66 | 0.971437 |
Target: 5'- cGGUUUcguaAGCGCUGCUGGCUuCGCUaucgcugcugGUGCu -3' miRNA: 3'- -UCAAA----UUGCGGCGGCUGA-GUGG----------CAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 93908 | 0.66 | 0.971437 |
Target: 5'- cGUaUGcCGCCauguuuGCCGugUCAUUGUGCa -3' miRNA: 3'- uCAaAUuGCGG------CGGCugAGUGGCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 176 | 0.67 | 0.965138 |
Target: 5'- gAGUUUGcgccugacGCGCCGCUG-UUCACUGg-- -3' miRNA: 3'- -UCAAAU--------UGCGGCGGCuGAGUGGCaug -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 45825 | 0.67 | 0.961649 |
Target: 5'- --gUUGGCGCCGCCGcC-CACguuggUGUACa -3' miRNA: 3'- ucaAAUUGCGGCGGCuGaGUG-----GCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 104702 | 0.67 | 0.961649 |
Target: 5'- gAGUccagcGCGCCGCCGuugUACgCGUACg -3' miRNA: 3'- -UCAaau--UGCGGCGGCugaGUG-GCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 112571 | 0.67 | 0.957924 |
Target: 5'- ----cAACGCCGUacuugcacaaUGGCgCGCCGUGCg -3' miRNA: 3'- ucaaaUUGCGGCG----------GCUGaGUGGCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 104059 | 0.67 | 0.957538 |
Target: 5'- uGUggccgUGGCGCCuucgucgugcuugGCCGGCUCGuuGUGa -3' miRNA: 3'- uCAa----AUUGCGG-------------CGGCUGAGUggCAUg -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 71506 | 0.67 | 0.953958 |
Target: 5'- ----cAACGCgGCCGACgacggUACCGgACa -3' miRNA: 3'- ucaaaUUGCGgCGGCUGa----GUGGCaUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 46056 | 0.67 | 0.953958 |
Target: 5'- aGGUU--AUGCCGCCG-CUgCACagCGUGCg -3' miRNA: 3'- -UCAAauUGCGGCGGCuGA-GUG--GCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 63412 | 0.68 | 0.949748 |
Target: 5'- gAGUUUGGCGCCGaaaaCGAa-CACCGc-- -3' miRNA: 3'- -UCAAAUUGCGGCg---GCUgaGUGGCaug -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 25852 | 0.68 | 0.948437 |
Target: 5'- ----aAGCGCCaacuacaacaacuuGUCG-CUCGCCGUGCa -3' miRNA: 3'- ucaaaUUGCGG--------------CGGCuGAGUGGCAUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 32349 | 0.68 | 0.940579 |
Target: 5'- ----aAACGCCGCCGug-CGCCG-ACc -3' miRNA: 3'- ucaaaUUGCGGCGGCugaGUGGCaUG- -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 20523 | 0.68 | 0.935613 |
Target: 5'- uGUUacACGCaCGCCGACUgGuuGUGg -3' miRNA: 3'- uCAAauUGCG-GCGGCUGAgUggCAUg -5' |
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22930 | 3' | -52 | NC_005137.2 | + | 50174 | 0.68 | 0.930392 |
Target: 5'- ----aAACGUCGaguCCGugUCGCCGUAg -3' miRNA: 3'- ucaaaUUGCGGC---GGCugAGUGGCAUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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