Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22938 | 3' | -54.7 | NC_005137.2 | + | 64161 | 0.66 | 0.905805 |
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Target: 5'- cCCGUGCgCGUUUaacggCGCCGGACAc -3' miRNA: 3'- aGGUACG-GCGAGaaca-GCGGCUUGUu -5' |
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| 22938 | 3' | -54.7 | NC_005137.2 | + | 6975 | 0.66 | 0.884797 |
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Target: 5'- gUCCAcgaUGCUGCgcacacggacgagcgGUCGCCGGGCAc -3' miRNA: 3'- -AGGU---ACGGCGagaa-----------CAGCGGCUUGUu -5' |
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| 22938 | 3' | -54.7 | NC_005137.2 | + | 49269 | 0.67 | 0.86307 |
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Target: 5'- cUCCGUGUCGCUgCUUGgCGCUuuGCGu -3' miRNA: 3'- -AGGUACGGCGA-GAACaGCGGcuUGUu -5' |
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| 22938 | 3' | -54.7 | NC_005137.2 | + | 6849 | 0.67 | 0.855138 |
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Target: 5'- gUCCGUGuuGCUaucgaacgUGUCGCUGGggcACAGc -3' miRNA: 3'- -AGGUACggCGAga------ACAGCGGCU---UGUU- -5' |
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| 22938 | 3' | -54.7 | NC_005137.2 | + | 63145 | 0.67 | 0.846164 |
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Target: 5'- aCCAUGgguCCGC-CgaaaaagaaagcgUUGUCGCCGAACGc -3' miRNA: 3'- aGGUAC---GGCGaG-------------AACAGCGGCUUGUu -5' |
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| 22938 | 3' | -54.7 | NC_005137.2 | + | 48737 | 1.07 | 0.003779 |
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Target: 5'- gUCCAUGCCGCUCUUGUCGCCGAACAAg -3' miRNA: 3'- -AGGUACGGCGAGAACAGCGGCUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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