Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22940 | 3' | -56.8 | NC_005137.2 | + | 2325 | 0.66 | 0.87119 |
Target: 5'- aACGCGcUCGUGgagGCGUGcAUGGcCGUa -3' miRNA: 3'- -UGCGC-AGCACag-CGCACaUGCC-GCAa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 51418 | 0.66 | 0.87119 |
Target: 5'- uACGUG-CGUGUCGUcguuuGUGUGCGGa--- -3' miRNA: 3'- -UGCGCaGCACAGCG-----CACAUGCCgcaa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 26809 | 0.66 | 0.87119 |
Target: 5'- uGCGuCGUCGUGUucgcgcugcgaCGCGcacgcuUGUACGaGCGUc -3' miRNA: 3'- -UGC-GCAGCACA-----------GCGC------ACAUGC-CGCAa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 85253 | 0.66 | 0.856045 |
Target: 5'- -gGCGUCGcUGUCGUca--GCGGCGUa -3' miRNA: 3'- ugCGCAGC-ACAGCGcacaUGCCGCAa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 31725 | 0.66 | 0.840086 |
Target: 5'- uCGCGUCGUuUCGCGUGcguauaGCcGCGUUc -3' miRNA: 3'- uGCGCAGCAcAGCGCACa-----UGcCGCAA- -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 17850 | 0.67 | 0.814744 |
Target: 5'- -gGCGUCGUGUCaaGCGUcggGUAUuGCGUUu -3' miRNA: 3'- ugCGCAGCACAG--CGCA---CAUGcCGCAA- -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 96493 | 0.68 | 0.740478 |
Target: 5'- uGCGCGUCGUGUCcacggGCGcuaUGacuaGCGGCGc- -3' miRNA: 3'- -UGCGCAGCACAG-----CGC---ACa---UGCCGCaa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 87251 | 0.69 | 0.710809 |
Target: 5'- -gGCGUUGUuaacgGcCGCGUGcACGGCGUUc -3' miRNA: 3'- ugCGCAGCA-----CaGCGCACaUGCCGCAA- -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 117138 | 0.69 | 0.680535 |
Target: 5'- cACGCGUCGcGUUG-GUGggaUGCGGCGg- -3' miRNA: 3'- -UGCGCAGCaCAGCgCAC---AUGCCGCaa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 102764 | 0.7 | 0.649899 |
Target: 5'- cACGUG-CGg--UGCGUGUugGGCGUUc -3' miRNA: 3'- -UGCGCaGCacaGCGCACAugCCGCAA- -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 63450 | 0.7 | 0.649899 |
Target: 5'- cGCGCGUUGUGccCGCGagcgACGGCGa- -3' miRNA: 3'- -UGCGCAGCACa-GCGCaca-UGCCGCaa -5' |
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22940 | 3' | -56.8 | NC_005137.2 | + | 56495 | 1.05 | 0.00375 |
Target: 5'- aACGCGUCGUGUCGCGUGUACGGCGUUa -3' miRNA: 3'- -UGCGCAGCACAGCGCACAUGCCGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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