miRNA: 3'- -AugCUUAGCC----ACCUGCGgCG----------CGUUGC- -5'
miRNA: 3'- -AugCUUAGCC---ACCugCG-GCGCGUUGc -5'
miRNA: 3'- -AUGCUUAGCCACCUGCGGCGCGUUGC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22941 | 5' | -55.7 | NC_005137.2 | + | 112438 | 0.73 | 0.520803 |
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Target: 5'- gUugGAGUCGGccguUGGACGCgGCggcaggauggGCGACGg -3' miRNA: 3'- -AugCUUAGCC----ACCUGCGgCG----------CGUUGC- -5' |
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| 22941 | 5' | -55.7 | NC_005137.2 | + | 11316 | 0.78 | 0.271669 |
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Target: 5'- gUugGGAUCGGgcgUGGugGCaCGCGCAGCc -3' miRNA: 3'- -AugCUUAGCC---ACCugCG-GCGCGUUGc -5' |
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| 22941 | 5' | -55.7 | NC_005137.2 | + | 57448 | 1.08 | 0.003358 |
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Target: 5'- gUACGAAUCGGUGGACGCCGCGCAACGa -3' miRNA: 3'- -AUGCUUAGCCACCUGCGGCGCGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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