Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22943 | 3' | -62.1 | NC_005137.2 | + | 26521 | 0.69 | 0.409513 |
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Target: 5'- -cGCGGCGCUGGCugcguuuugcGUGcCGGU-AGCg -3' miRNA: 3'- caCGCCGCGGCCG----------CACaGCCGuUCGa -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 70159 | 0.69 | 0.384632 |
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Target: 5'- -gGCGGUGUCGGUgaagGUGUUGGCGucGGUg -3' miRNA: 3'- caCGCCGCGGCCG----CACAGCCGU--UCGa -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 124963 | 0.7 | 0.353052 |
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Target: 5'- -cGCGGCGCCGGUGaUGg-GGCGcuGCg -3' miRNA: 3'- caCGCCGCGGCCGC-ACagCCGUu-CGa -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 31030 | 0.71 | 0.309215 |
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Target: 5'- cUGCGGCGCauuGGCGUucgGUCGuGCAcGCUu -3' miRNA: 3'- cACGCCGCGg--CCGCA---CAGC-CGUuCGA- -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 35515 | 0.71 | 0.295554 |
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Target: 5'- -aGCGGCGCCuGCGUguuguuggucggGUCGGCuucguaauAAGCUa -3' miRNA: 3'- caCGCCGCGGcCGCA------------CAGCCG--------UUCGA- -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 55567 | 0.73 | 0.234269 |
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Target: 5'- -gGCGGCGCCGuGCGcGUgGaGCAAGUUg -3' miRNA: 3'- caCGCCGCGGC-CGCaCAgC-CGUUCGA- -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 48782 | 0.73 | 0.228766 |
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Target: 5'- -cGCGGCGCCGcacaGCGUGUgCGGCAcuacauuGCg -3' miRNA: 3'- caCGCCGCGGC----CGCACA-GCCGUu------CGa -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 96456 | 0.83 | 0.04575 |
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Target: 5'- uUGC-GCGCCGGCGUGUCGGCGAauGCa -3' miRNA: 3'- cACGcCGCGGCCGCACAGCCGUU--CGa -5' |
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| 22943 | 3' | -62.1 | NC_005137.2 | + | 63012 | 1.06 | 0.001012 |
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Target: 5'- gGUGCGGCGCCGGCGUGUCGGCAAGCUu -3' miRNA: 3'- -CACGCCGCGGCCGCACAGCCGUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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