Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22947 | 5' | -53 | NC_005137.2 | + | 39292 | 0.66 | 0.947581 |
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Target: 5'- -aCAcgUCGCgCGCAAaaAGUGCGUCGu -3' miRNA: 3'- ugGUuuGGCGgGCGUU--UCACGUAGUu -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 86782 | 0.66 | 0.947581 |
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Target: 5'- cGCCAAcCCGUUCGCcGAG-GCGUUGAa -3' miRNA: 3'- -UGGUUuGGCGGGCGuUUCaCGUAGUU- -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 78692 | 0.66 | 0.942922 |
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Target: 5'- cGCUGAGcCCGCguaGCAAAGUGCAUUu- -3' miRNA: 3'- -UGGUUU-GGCGgg-CGUUUCACGUAGuu -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 113008 | 0.67 | 0.911959 |
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Target: 5'- gACCGAGCCGCagcgaugCGCGAaggcuuugaaaacgaGGUGCAaUCAGc -3' miRNA: 3'- -UGGUUUGGCGg------GCGUU---------------UCACGU-AGUU- -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 107639 | 0.67 | 0.90943 |
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Target: 5'- uGCCcGACCGCaCgGCGuuuaAAGUGCAaaUCAAc -3' miRNA: 3'- -UGGuUUGGCG-GgCGU----UUCACGU--AGUU- -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 110558 | 0.68 | 0.858604 |
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Target: 5'- cGCCGauGugCGCCCGCAcc-UGCGUCu- -3' miRNA: 3'- -UGGU--UugGCGGGCGUuucACGUAGuu -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 77376 | 0.72 | 0.674028 |
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Target: 5'- aACUGGACCGCuuGCAacGAGUGCGcCAc -3' miRNA: 3'- -UGGUUUGGCGggCGU--UUCACGUaGUu -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 95588 | 0.72 | 0.652548 |
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Target: 5'- cGCCGuuuuGCCGCCCGCGcacuGUGCG-CGAc -3' miRNA: 3'- -UGGUu---UGGCGGGCGUuu--CACGUaGUU- -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 95427 | 0.74 | 0.577287 |
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Target: 5'- uGCUcguuACCGUCCGCAucGUGCAUCu- -3' miRNA: 3'- -UGGuu--UGGCGGGCGUuuCACGUAGuu -5' |
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| 22947 | 5' | -53 | NC_005137.2 | + | 73481 | 1.06 | 0.006063 |
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Target: 5'- cACCAAACCGCCCGCAAAGUGCAUCAAc -3' miRNA: 3'- -UGGUUUGGCGGGCGUUUCACGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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