Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22950 | 3' | -58.7 | NC_005137.2 | + | 81681 | 1.06 | 0.002091 |
Target: 5'- gAAUCGCUGCCGGUGUCGGGCGGAACAc -3' miRNA: 3'- -UUAGCGACGGCCACAGCCCGCCUUGU- -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 71079 | 0.76 | 0.227306 |
Target: 5'- aAAUUGCUGCaaaucaaUGUCGGGCGGAGCGu -3' miRNA: 3'- -UUAGCGACGgcc----ACAGCCCGCCUUGU- -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 108124 | 0.69 | 0.553791 |
Target: 5'- cGAUCGCaGCaccgcauccCGGUcGUCGGGCGGcGGCGg -3' miRNA: 3'- -UUAGCGaCG---------GCCA-CAGCCCGCC-UUGU- -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 124986 | 0.67 | 0.706228 |
Target: 5'- --gCGCUGCguUGGaaaUCGGGCGGGAUg -3' miRNA: 3'- uuaGCGACG--GCCac-AGCCCGCCUUGu -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 23042 | 0.66 | 0.726117 |
Target: 5'- -cUCGCUGgaGGUGUucacCGuGGCGGAcgACAa -3' miRNA: 3'- uuAGCGACggCCACA----GC-CCGCCU--UGU- -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 44102 | 0.66 | 0.764847 |
Target: 5'- uAUCGCUGCUGGUGcUGGacGCGuGggUg -3' miRNA: 3'- uUAGCGACGGCCACaGCC--CGC-CuuGu -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 73949 | 0.66 | 0.764847 |
Target: 5'- aGAUCGCUGCCGGUcagaauuaaCGcGGUGGucacguACAa -3' miRNA: 3'- -UUAGCGACGGCCAca-------GC-CCGCCu-----UGU- -5' |
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22950 | 3' | -58.7 | NC_005137.2 | + | 19806 | 0.66 | 0.726117 |
Target: 5'- --aUGCUGCUGGUagCGGGCGcAACGc -3' miRNA: 3'- uuaGCGACGGCCAcaGCCCGCcUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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